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Journal of Chemistry
Volume 2013, Article ID 946248, 7 pages
Review Article

Parameters Affecting the Performance of Immobilized Enzyme

1College of Pharmacy, Hebei University, Baoding 071002, China
2Key Laboratory of Pharmaceutical Quality Control of Hebei Province, College of Pharmacy, Hebei University, Baoding 071002, China

Received 10 May 2013; Accepted 2 August 2013

Academic Editor: Yufang Zhu

Copyright © 2013 Dong-Hao Zhang et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.


Enzyme immobilization has been investigated to improve lipase properties over the past few decades. Different methods and various carriers have been employed to immobilize enzyme. However, the application of enzymatic technology in large scale is rarely seen during the industrial process. The main obstacles are a high cost of the immobilization and the poor performance of immobilized lipase. This review focuses on the current status of enzyme immobilization, which aims to summarize the latest research on the parameters affecting the performance of immobilized enzyme. Particularly, the effect of immobilization methods, immobilization carriers, and enzyme loading has been discussed.