Bhanupaka: A Green Process in the Preparation of an Indian Ayurvedic Medicine, Lauha Bhasma

Table 1

Experimental conditions used for LC-MS/MS analysis of Triphala decoction.

Column	RP C18 (100 mm × 3.9 mm); internal diameter = 5 𝜇m
Elution	Gradient
Mobile phases
A	Acetonitrile
B	MilliQ water with acetic acid (2%)
Gradient profile
0–10 min	95% B and 5% A
10-11 min	90% B and 10% A
11–20 min	80% B and 20% A
20-21 min	90% B and 10% A
21 min—end of run	100% A
Flow rate	800 𝜇L/min
Detection wavelength	355 nm
Mass spectra	Negative ion mode
Fragmentor voltage (V)	100
Capillary voltage (V)	2500
Nebulizer pressure (psi)	30.5
Drying gas temperature (°C)	350
𝑚/𝑧 scan range	100–1500
Gas flow (L/min)	6.0
Collision energy (eV)	8
Retention Factor (Vpp)	150
Transfer time (𝜇s)	80
Pre pulse storage (𝜇s)	10