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Journal of Chemistry
Volume 2014, Article ID 248065, 9 pages
Research Article

Separation/Preconcentration and Speciation Analysis of Trace Amounts of Arsenate and Arsenite in Water Samples Using Modified Magnetite Nanoparticles and Molybdenum Blue Method

1Department of Chemistry, Payame Noor University, P.O. Box 19395-4697, Tehran, Iran
2Department of Chemistry & Nanoscience and Nanotechnology Research Laboratory (NNRL), Payame Noor University, Sirjan, P.O. Box 78185-347, Iran
3Department of Chemistry, Payame Noor University, Kerman, Iran

Received 4 May 2013; Revised 16 November 2013; Accepted 17 November 2013; Published 2 March 2014

Academic Editor: Daryoush Afzali

Copyright © 2014 Mohammad Ali Karimi et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.


A new, simple, and fast method for the separation/preconcentration and speciation analysis of arsenate and arsenite ions using cetyltrimethyl ammonium bromide immobilized on alumina-coated magnetite nanoparticles (CTAB@ACMNPs) followed by molybdenum blue method is proposed. The method is based on the adsorption of arsenate on CTAB@ACMNPs. Total arsenic in different samples was determined as As(V) after oxidation of As(III) to As(V) using potassium permanganate. The arsenic concentration has been determined by UV-Visible spectrometric technique based on molybdenum blue method and amount of As(III) was calculated by subtracting the concentration of As(V) from total arsenic concentration. MNPs and ACMNPs were characterized by VSM, XRD, SEM, and FT-IR spectroscopy. Under the optimal experimental conditions, the preconcentration factor, detection limit, linear range, and relative standard deviation (RSD) of arsenate were 175 (for 350 mL of sample solution), 0.028  g mL−1, 0.090–4.0  g mL−1, and 2.8% (for 2.0  g mL−1, ), respectively. This method avoided the time-consuming column-passing process of loading large volume samples in traditional SPE through the rapid isolation of CTAB@ACMNPs with an adscititious magnet. The proposed method was successfully applied to the determination and speciation of arsenic in different water samples and suitable recoveries were obtained.