Pharmacokinetic Comparison of Isoalantolactone and Alantolactone in Rats after Administration Separately by Optimization of an UPLC-MS² Method

Figure 2

Representative multiple reaction monitoring chromatograms of (a) a blank plasma sample of rats, (b) a blank rat plasma sample spiked with IS (0.1 ng/mL), (c) a blank rat plasma sample spiked with lactones ((c1) for isoalantolactone at 2.5 ng/mL and (c2) for alantolactone at 4 ng/mL), (d) acetonitrile spiked with IS and lactones ((d1) for isoalantolactone and (d2) for alantolactone), and (e) a rat plasma sample obtained 2 h after intravenous injection of 5 mg/kg isoalantolactone (e1) and 4 h of alantolactone (e2).

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