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Journal of Chemistry
Volume 2014 (2014), Article ID 385908, 6 pages
Research Article

Application of HPLC to Study the Reaction of Free Radicals with Antioxidants and/or Toxins

Department of Analytical Chemistry, Faculty of Science, Institute of Chemistry, Siedlce University of Natural Sciences and Humanities, 3 Maja 54, 08-110 Siedlce, Poland

Received 16 May 2013; Revised 22 December 2013; Accepted 5 January 2014; Published 13 February 2014

Academic Editor: Aicheng Chen

Copyright © 2014 Paweł Piszcz et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.


The application of HPLC in the antioxidative (antiradical) properties studies of various samples is discussed in this paper. The assay is based on the reaction of, characterized by strong oxidizing properties, hydroxyl radicals (generated in the Fenton-like reaction) with the sample and with the, so-called, sensor. The product of the sensor reaction with the radicals is analyzed using RP-HPLC with fluorescence detection. It is well known that antioxidants, which are healthy for living organisms, have a negative environmental effect. Therefore, the goal of the paper is to discuss if the same setup can be used to get rid of unwanted compounds (toxins) from the environment. It was found that the phenols and PAHs are degraded by hydroxyl radicals. The optimal (maximum degree of the degradation) conditions were obtained for and 0.1 mM Fe2+.