Research Article
Application of an Optimized HPLC Method for the Detection of Various Phenolic Compounds in Apples from Lithuanian Cultivars
Table 1
Characteristics of the quantitative evaluation of phenolic compounds.
| | Compound | Retention time (min)a | Wavelength (nm) | Confirmed linearity range (µg/mL) | Calibration equationb | Determined quantitation limit (µg/mL) | |
| | Procyanidin B1 | 15.6 | 280 | 6.25–100 | y = 7.33 × 103x + 5.49 × 103 | 2.64 | 0.9998 | | (+)-Catechin | 19.7 | 280 | 5–80 | y = 7.00 × 103x − 1.87 × 103 | 2.20 | 0.9999 | | Chlorogenic acid | 20.7 | 320 | 6.25–100 | y = 2.68 × 104x − 3.02 × 104 | 2.93 | 0.9999 | | Procyanidin B2 | 22.9 | 280 | 6.25–100 | y = 5.58 × 103x + 7.15 × 103 | 2.86 | 0.9998 | | (−)-Epicatechin | 27.3 | 280 | 5–80 | y = 7.53 × 103x + 1.19 × 102 | 2.22 | 0.9999 | | Hyperoside | 40.0 | 360 | 5–80 | y = 2.23 × 104x − 1.01 × 104 | 2.42 | 0.9999 | | Isoquercitrin | 40.1 | 360 | 3.125–50 | y = 2.66 × 104x − 2.10 × 104 | 1.54 | 0.9999 | | Rutin | 39.5 | 360 | 3.125–50 | y = 1.47 × 104x − 7.66 × 103 | 2.79 | 0.9998 | | Quercitrin | 44.5 | 360 | 2.5–40 | y = 1.77 × 104x − 5.57 × 103 | 2.19 | 0.9998 | | Avicularin | 43.9 | 360 | 3.125–50 | y = 2.32 × 104x − 1.12 × 104 | 2.56 | 0.9998 | | Phloridzin | 47.6 | 280 | 3.125–50 | y = 1.85 × 104x − 7.09 × 103 | 2.40 | 0.9998 |
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The mean was calculated from 6 replicates.
x
in the calibration equation indicates the concentration of a compound, and y indicates a peak area.
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