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Journal of Chemistry
Volume 2014 (2014), Article ID 580418, 5 pages
http://dx.doi.org/10.1155/2014/580418
Research Article

Simultaneous Determination of Florfenicol and Diclazuril in Compound Powder by RP-HPLC-UV Method

1School of Pharmacy, Xinxiang Medical University, Jin Sui Road, Xinxiang 453003, China
2Medicinal Experiment Center, Xinxiang Medical University, Jin Sui Road, Xinxiang 453003, China

Received 2 April 2014; Revised 16 May 2014; Accepted 24 May 2014; Published 12 June 2014

Academic Editor: Pranav S. Shrivastav

Copyright © 2014 Leilei Guo et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

A RP-HPLC-UV method was developed and validated for simultaneous determination of florfenicol and diclazuril in compound powder. The separation involved using a SinoChoom ODS-BP C18 (5 μm, 4.6 mm × 250 mm) analytical column. The mobile phase was a mixture of acetonitrile-0.2% phosphoric acid (pH was adjusted to 3.0 with triethylamine). The ratio of acetonitrile and 0.2% phosphoric acid in the mobile phase was 60 : 40 (v/v) from 0 minutes to 6 minutes and 70 : 30 (v/v) from 6.1 minutes to 15 minutes. The flow rate was 1 mL/min. The temperature of the analytical column was maintained at 30°C. The detection was monitored at 225 nm and 277 nm for florfenicol and diclazuril, respectively. The excipients in the compound powder did not interfere with the drug peaks. The calibration curves of florfenicol and diclazuril were fairly linear over the concentration ranges between 50.0–500.0 μg/mL ( ) and 10.0–100.0 μg/mL ( ), respectively. The RSD of both the intraday and interday variations was below 2.1% for florfenicol and diclazuril. The method was successfully validated according to International Conference on Harmonisation and proved to be suitable for the simultaneous determination of florfenicol and diclazuril in compound powder.