Synthesis of New [1,2,4]Triazolo[3,4-b][1,3,4]thiadiazines and Study of Their Anti-Candidal and Cytotoxic Activities

Table 3

In vitro cytotoxicity of compounds (5a–h) on noncancer cell line (MCF-12).

Compound	IC50 (µg/mL)^a
5a	>1.0 × 10^5
5b	3.0 × 10^4
5c	6.6 × 10^4
5d	>1.2 × 10^5
5e	5.4 × 10^4
5f	5.4 × 10^4
5g	2.4 × 10^4
5h	>1.0 × 10^5
Gentamicin	>1.0 × 10^5
Ketoconazole	>1.0 × 10^5

aIC₅₀: concentration of the compound (𝜇g/mL) producing 50% cell growth inhibition after 48 h of compound exposure, as determined by the WST-1 assay. Each experiment was run at least three times, and the results are presented as average values.