HPLC system Mobile phase Column Detection mode Matrix Remarks Reference Ag-HPLC Isocratic solvent system: hexane-acetonitrile 99.7 : 0.3 (v/v) Chromspher 5 Lipid column ( UV-VIS detector Deodorized fish oil containing EPA, DHA, GLA, LA, ALA, SD, and other PUFA Detection levela [63 ] HPLC-ECD Ethanol-acetonitrile 20 : 80 (v/v) ODS 100RP-18 column, Electrochemical detector, saturated calomel electrode, detection potential −415 mV Human plasma, containing various fatty acids including arachidonic acid and linoleic and oleic acids Detection level: 50–1600 pmoles [80 ] HPLC-MS-APCI The gradient solvent program was used: MeCN/EtCN/DCM Hichrom column HIRPB-250 AM, 250 mm × 2.1 mm, 5 Picolinyl esters were separated by use of quadruple mass spectrometer fitted with an atmospheric pressure chemical ionization source Dinoflagellate Amphidinium carterae containing VLC-PUFA up nonacosahexaenoic acid Detection levela [81 ] RP-HPLC-ELSD The isocratic elution of MeCN/H2 O was used: 97.5 : 2.5 (v/v) flow rate of mobile phase 1.5 ml/min RP column (Alltima, C18, Alltech, 250 mm × 4.6 mm), 5 Methyl esters PUFA were detected by evaporative light-scattering detector (ELSD) Marine algae Schizochytrium sp. containing DHA, EPA, and their derivatives Detection level: mg [82 ] Ag-HPLC Dichloromethane-acetonitrile 100 : 0.025 (v/v) Nucleosil 100-5SA UV-VIS detector, the working wavelength was 250 nm for the anthrylmethyl and naphthyl derivatives and also 275 nm for the naphthylmethyl derivatives of examined octadecenoic acid Reference standards of positional isomers of octadecenoic acid and their derivatives (esters) Detection level: [83 ] Ag-HPLC Gradient solvent system consists of hexane-isopropanol-acetonitrile Chrompack 5 Lipid column (250 mm × 4.6 mm, Netherlands) UV-VIS detector, Oil produced by a marine microbe containing PUFA (DHA, EPA) Detection levela [84 ]
