Antioxidant and Chelating Activity of Nontoxic Jatropha curcas L. Protein Hydrolysates Produced by In Vitro Digestion Using Pepsin and Pancreatin

Figure 6

Antioxidant activity of increasing concentrations of J. curcas hydrolysates in Caco-2 cell cultures treated with the free radical generator ABAP. Formation of reactive oxygen species was determined by oxidation of the fluorescent dye DCFH. Antioxidant effect of hydrolysate produced by treatment solely with pepsin for 60 minutes (a) or treatment with pepsin for 60 minutes followed by treatment with pancreatin for 120 minutes (b). (c) FPLC fractions C, D, and E (50 μg protein) from 180 min hydrolysate. Values are the mean of data from five wells ± standard deviation. Experiments were conducted in duplicate on two different days. Different letters indicate significant differences ().

(a)

(b)

(c)