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Journal of Chemistry
Volume 2015, Article ID 384051, 6 pages
Research Article

Improvement of Analytical Methods for the Determination of Polyphenolic Bioactive Compounds in Berry Fruits

1School of Agriculture, Forestry, Food and Environmental Science, University of Basilicata, Viale dell’Ateneo Lucano 10, 85100 Potenza, Italy
2Department of Biotechnology, University of Verona, Ca’ Vignal 1, Strada Le Grazie 15, 37134 Verona, Italy

Received 24 January 2015; Revised 27 May 2015; Accepted 4 June 2015

Academic Editor: Ghada M. Hadad

Copyright © 2015 Marisa Carmela Caruso et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.


Berry fruits contain high levels of different phytochemicals, most of which are phenolic molecules. Fruits of the same cultivar from different locations and different harvest years have different chemical compositions, particularly related to polyphenols. The difference may be due to specific climatic conditions, the type of soil in which the plants grow, and the stresses to which the plants were subjected because these phytochemicals are produced as a defense mechanism through a secondary metabolic process. For this reason, it is important to establish simple and reliable procedure to determine polyphenolic compounds in berry fruits considering the increasing attention on these compounds for different potential uses. In order to choose and to improve the most adequate analytical procedure for the determination of the polyphenolic substances in berry fruits, different methods were applied and compared on samples of elderberry and blackberry.