Improvement of Analytical Methods for the Determination of Polyphenolic Bioactive Compounds in Berry Fruits

Table 1

Content of anthocyanins in the traditional and ultrasonic extracts (results from 3 independent replicates).

Sample	Analyte	Methanolic extract		Methanolic extract with ultrasound treatment
		Concentration; (mg 100 g^−1 FW)^a	RSD; (%)	Concentration; (mg 100 g^−1 FW)^a	RSD; (%)
S1 (S. nigra)	Cy-3-glucoside	201.22	8.18	209.48	13.84
	Cy-3-sambubioside	271.43	12.22	285.95	6.69
S2 (S. nigra)	Cy-3-glucoside	188.21	5.34	179.76	7.94
	Cy-3-sambubioside	220.46	11.22	232.74	12.08
S3 (S. nigra)	Cy-3-glucoside	179.13	8.45	156.70	7.15
	Cy-3-sambubioside	339.14	4.41	353.27	6.39
M1 (R. ulmifolius)	Cy-3-glucoside	33.03	3.57	36.06	5.32
	A1	n.d.	—	n.d.	—
	A2	44.68	6.88	49.94	4.40
	A3	45.84	5.34	42.67	4.40
M2 (R. ulmifolius)	Cy-3-glucoside	16.98	8.22	18.82	6.00
	A1	n.d.	—	n.d.	—
	A2	29.44	2.37	27.32	6.44
	A3	29.88	7.87	32.52	6.12
M3 (R. procerus)	Cy-3-glucoside	24.58	9.98	20.21	8.66
	A1	22.84	7.84	26.96	7.04
	A2	29.32	4.22	26.99	5.70
	A3	25.49	6.77	33.31	6.04
M4 (R. fruticosus)	Cy-3-glucoside	151.88	3.54	177.67	5.43
	A1	21.84	9.22	23.07	2.90
	A2	28.55	7.55	26.04	4.22
	A3	31.18	6.49	29.96	3.44

RSD: relative standard deviation; ^amean value; n.d.: not detected.