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Journal of Chemistry
Volume 2015 (2015), Article ID 414237, 9 pages
Research Article

Removal of Ethanethiol Gas by Iron Oxide Porous Ceramsite Biotrickling Filter

1Jiangsu Key Laboratory of Atmospheric Environment Monitoring and Pollution Control, School of Environmental Science and Engineering, Nanjing University of Information Science and Technology, Nanjing 210044, China
2School of Resources & Environmental Engineering, Hefei University of Technology, Hefei 230009, China

Received 22 September 2014; Accepted 16 December 2014

Academic Editor: Jian Lu

Copyright © 2015 Peipei Wang et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.


The performance of ethanethiol removal in biotrickling filter was investigated by microorganisms fixed on iron oxide-based porous ceramsite (IPC) under different operating parameters conditions. Ethanethiol removal efficiency was examined as a function of inlet concentration, empty bed residence time (EBRT), and spray density of nutrient solution. The results showed that the optimized operation conditions and operation characteristics of biotrickling filter for this study were at the inlet concentration of less than 250 mgm−3, the spray density of 0.24 m3m−2 h−1, and the EBRT of 68.7 s. The variation of the EBRT of about 100 s and the spray density of about 0.24 m3m−2 h−1 did not change the ethanethiol removal efficiencies at certain ethanethiol concentrations of less than about 300 mg/m3, respectively. The main metabolic product was sulfate such as under continuous long-running regime in filter. The ethanethiol desulfurization process better meets the Michaelis-Menien model with calculated kinetic degradation parameters  mgm−3 and  gm−3 h−1.

1. Introduction

Ethanethiol (ET, CH3-CH2-SH) is volatile organic sulfur compounds (VOSCs) with an intensive garlic odour; it is often used as malodorant and warning agent and is a significant organophosphorus pesticide intermediate [1, 2]. ET is usually released from various industries and can cause different kinds of environmental problems. And when it functioned in the central nervous system of human body, it could cause poisoning, headache, and nausea and even lead to death due to respiratory paralysis [3]. Therefore, it is desirable and catches many people’s growing concern to develop new technologies or improve existing treatment technologies to control such VOSCs like ET nowadays. Lots of physical and chemical abatement technologies, such as combustion method [46], oxidation process [79], and absorption method [10, 11], have been invented for purifying VOSCs. But their performance is not satisfying and they do not really degrade VOCs. By some traditional physical technologies, VOCs may just be transferred from gaseous phase to other phase [12]. Moreover, the chemical technologies are generally much more expensive [4]. However, the biological technologies have been reported by some research to be a promising effective alternative for desulfurization processes for removal of VOSCs with low operating costs, low operating requirements, and no by-products produced for disposal treatment moreover, they can process simultaneously two or more contaminants [1316]. Biotrickling filter and biofiltration processes are two sorts of biological desulfurization process that have been widely applied [12, 1418]. And compared with other biological methods, biotrickling filter process was proved to have an advantage of higher rates of mineralization [12, 19].

In recent years, the biological treatment study of VOCs such as ethanethiol could be mainly summarized about these respects, the research for advantage microbial strains cultivation and living conditions optimization, the designation and optimization of the biological reactor, as well as the biological filter, and the research for biodegradation dynamics. For example, An et al. [12, 19] had inoculated with Lysinibacillus sphaericus strain RG-1 or RG-1 and B350 mixed microorganisms in biotrickling filter columns to remove ethanethiol and single, binary, and ternary mixture gas of ethanethiol, dimethyl disulfide, and thioanisole. However, both researches had got satisfying results. And with the continuous development of anaerobic technology [2022], Arellano-García et al. [23] had used an aerobic biotrickling filter system for removal of waste gas containing ethanethiol from their published paper. In addition, Sedighi and Vahabzadeh [24] studied the kinetic modeling of cometabolic degradation of ethanethiol. Hernández et al. [25] had used biotrickling filters packed with poplar wood and polyurethane foam to remove simultaneously H2S, NH3, and ET and investigate the impact of pH during startup and crossed effects evaluation.

In present study, biotrickling filter system was employed in this study for the purpose of removal of target gas, ethanethiol. Organic sulfide was absorbed by microorganism fixed on packing materials (usually Thiobacillus such as T. denitrificans, T. thioparus, and others) and then degraded and transformed to inorganic sulfate or elemental sulfur [2630]. The effects of the filter performance with different operating parameters such as inlet concentration spray amount and empty bed residence time (EBRT) in the stage of start-up and stable-running were systematic investigated. In addition, dynamic analysis for this biological desulfurization process was also preliminarily discussed. All of the research contents would provide an effective technical reference for further study and application about biotrickling filter.

2. Materials and Methods

2.1. Experimental Set-Up and Technological Process

Using multilayer filler could improve the treatment efficiency and stability of filtration column because of the decreased relative thickness of each layer which would reduce the weight of filler of each layer and lead the filler difficultly to compact. While air flow was redistribute through each layer, so that the gas flow distribution could be more uniform, the flow rate could decrease and the residence time could be longer. Huaxin et al. [31] used natural corncob and sawdust as filter and divided the column into three layers to remove H2S; the results showed that, in the test inlet concentration, a multilayer filter had a slight advantage over a single layer filter for the removal of H2S. However, the stabilization and the removal efficiency of biofilters were also improved. Chen et al. [32] used three pilot-scale immobilized-cell biotrickling filters for removal of hydrogen sulfide from a contaminated air steam and got a good performance. In present study, the biotrickling filter was a self-made plexiglass column (Figure 1) divided into three layers with filter inner diameter of 90 mm and the total reactor height of 1200 mm. Each layer was packed with 200 mm height iron oxide-based porous ceramsite (IPC) with particle size determined of about 5–8 mm. And the mud content was about 0.27%, hydrochloric acid soluble rate was approximately 0.43%, porosity was 71.48%, surface area was 7.1 × 105 cm2·g−1, a bulk density was 0.46 g·mL−1, an apparent density was 1.71 g·cm−3, ceramic strength was about 51–78 N, and porosity ratio was about 40.5–64.3%. The biotrickling filter was employed with up-flow running mode, a countercurrent operation [33]. Ethanethiol gas entered from the bottom of the filter, through three IPC filler layers, and then outflew from the top. However, circulating nutrient solution was totally opposite, sprayed from the top to the bottom lifted to reservoir tank by water pump (BQ5Q1-1J peristaltic pump, Baoding Longer Pump). And in order to sample output gas there was a sampling port in each layer.

Figure 1: Biotrickling filter set-up and technological process (1: liquid-flow meter; 2: sprinklers; 3: absorption bottle; 4: air pump; 5: ethanethiol reservoir; 6: peristaltic pump; 7: mixing gas tank; 8: compressor; 9: nutrient recirculation tank; 10: biotrickling filter; 11 and 13: gas-flow meter; 12: buffered gas-bottle; S, A, B, C, T: sampling port).

Certain concentration of ET was prepared by the method of dynamic preparation method. In other words, a small amount of air was bubbled into liquid ET solution in the ethanethiol reservoir to produce the pure ET gas, then the ET gas entered into mixing gas tank and mixed with air in order to form a certain concentration gas. Considered environmentally, the exhaust gas compound flew through the biotrickling filter was finally absorbed with 32% NaOH.

2.2. Analytical Methods

The concentrations of ET were analyzed by the amino dimethyl aniline spectrophotometry (GBZ/T160.49-2004) [34]. And the performance of studied biotrickling filter was generally evaluated by where (mg·L−1) is the inlet concentrations and (mg·L−1) is the outlet concentrations of gas compounds. However, the various inlet loads (g·m−3 h−1) calculated by (2) are also a significant parameter evaluating the filter performance:where (m3·h−1) is gas volume flow of gas compounds of ET and (m3) is the volume of studied biotrickling filter. In addition, empty bed residence time EBRT (s) and spray density (m3·m−2 h−1) as two necessary parameters can calculated by where (s) is EBRT and and in (3) are same as defined above in (2); (m3·h−1) and (m) in (4) are liquid spray flow and inner diameter of filter, respectively.

The energy of microbial growth metabolism provided by only ethanethiol in the process of the biodegradation of ethanethiol occurred in studied filter [30]. Because of the biodegradation by microorganism, organic sulfate (ET) could be transformed to inorganic sulfate or elemental sulfur such as , which led the nutrient solution change spontaneously into acidification. If ET can be completely degraded, the reaction is as follows [12]:

In that case the concentration of was determined through spectrophotometric method (GB/T13025.8-91) [35]. The microbiological quality fixed on inorganic packing surface was observed by using Scanning Electron Microscope (SEM, XL30 ESEM-TMP).

3. Results and Discussions

3.1. Biotrickling Filter Start-Up and Running Performance

Biotrickling filter started and run at certain conditions in which the experimental temperature was 20°C–25°C, the inlet concentration of ET was about 47.15–434.28 mg·m−3, the spray density was about 0.08–0.31 m3·m−2 h−1, and EBRT was 60 s after inoculating tame activated sludge (Zhu Zhuanjing sewage treatment plants of Hefei, Oxidation ditch). The performance of biological removal of ET was presented in Figure 2 after 26 d.

Figure 2: The ethanethiol inlet and outlet concentration and removal efficiency in start-up period.

Figure 2 showed that the ET removal efficiencies were 48.10%, 55.65%, and 61.45% on the 1st d, 4th d, and 12th d, respectively, with concentration of ET less than 251.24 mg·m−3. The removal efficiency stabilized at around 60% after a 18 days’ steady running of biotrickling filter with increased ET inlet concentration. However, 58.9% removal efficiency was detected on 26th d. The biofilm biomass was also determined about 0.034 g·g−1. And a white membranous substance attached to packing materials was observed in filter by naked eye. It was also found that larger ellipsoid bacteria with dimensions of about 18 × 38 μm with scaly structure are present in packing materials surface. From the SEM results (Figures 3(a) and 3(b)), the scaly structure had a serrated opening at one end. In addition, the substances immobilized on the surface of IPC filler were scraped to be observed under the microscope; the photo is shown in Figures 3(c) and 3(d). The freedom movement of rod-shaped microorganism could be found obviously in the observation. In a word, biotrickling filter start-up of inoculated operation completed basically after 26 d.

Figure 3: SEM images (a, b) and microscope photo (c, d) of IPC ceramsite after microbial inoculation.
3.2. Effect of Inlet Concentration on RE

In any biofiltration desulfurization process the initial concentration was a significant parameter on RE [27]. To study the effect of inlet concentration ranging from 100 to 500 mg·m−3 on RE in filter, experiment operation condition was controlled at a temperature of about 20–25°C, spray density of 0.24 m3·m−2 h−1, and EBRT of 60 s. The results were shown in Figure 4. It can be seen that the RE of ET was reduced when the inlet concentration increased. 63.98% RE obtained decreased into 57.39% when the inlet concentration increased from 95.74 to 501.39 mg·m−3. While the inlet concentrations were less than 250 mg·m−3, the removal efficiencies of ET were about 64% and the magnitude of decreasing of RE was smaller. In the study the maximum inlet load of the biotrickling filter was 34.22 g·m−3 h−1 and the actual maximum oxygen consumption was 88.30 g under the current biosystem according to the stoichiometric degradation reaction. However, the experiment condition of air in the biotrickling filter can provide more than 150 g oxygen. Thus, oxygen is sufficient for the microorganism to degrade ET in this biosystem. And a higher concentration gradient of ET could be produced by increasing inlet concentration, which would improve gaseous biofilm mass transfer rate from liquid phase to gas phase [12]. But the reason of decreased RE of ET in this study probably could be summarized as reaction substances constraints or diffusion limitation regime [26]. In addition, as the initial ethanethiol concentration increases, more time is needed to achieve the same RE because the lag phase of bacteria may be extended at higher concentrations of substrate [36]. However, biomass accumulation may be enhanced due to increase the gaseous compounds of ET inlet concentration with the various inlet loads changed from 3.76 to 19.72 g·m−3 h−1 in the process, so that the thickness of the biofilm could increase synchronously. As a result, the gas flow was obstructed because the porosity of packing materials reduced which led to decrease of biological activity [15].

Figure 4: The effects of inlet concentration on ethanethiol removal efficiency.
3.3. Effect of EBRT on RE

EBRT was also a significant parameter of any biofiltration desulfurization process [12] and the effect of EBRT was studied at experimental condition where temperature was about 20–25°C and spray density was 0.24 m3·m−2 h−1 in biotrickling filter (Figure 5). The results indicated that the removal performance could improve through increasing EBRT of biotrickling filter. Compared to three different inlet concentrations of effect of EBRT on RE, it could be seen a higher RE value when the ET inlet concentration was 200 mg·m−3 or 300 mg·m−3 than 400 mg·m−3, and the RE value of group (a) and (b) was of similar at lower EBRT. When EBRT increased to about 70 s an obviously increased trend of RE was achieved with comparison value of three group, 200 mg·m−3 > 300 mg·m−3 > 400 mg·m−3. In addition, better performance of RE was found at low concentration than high concentration of ET.

Figure 5: The effects of EBRT on ethanethiol removal efficiency.

As 3.2 described, ET biological degradation reaction was mainly controlled by reaction substances constraints or diffusion limitation regime. The reason for higher RE values in longer EBRT condition was that the increased EBRT in the diffusion process which gas molecules transfer from gas phase to biofilm surface could provide sufficient time of this diffusion process to degrade contaminants by biological flora [12]. The diffusion of gas molecules restricted under shorter EBRT condition because the biofilm could not deal with excess gas molecules with enough react time that limited the total removal efficiency of filter [26]. And when EBRT added to a certain extent the changing curve trend of RE began to be smooth due to the limitation of microbial degradation [3]. Compared with the RE of three inlet concentrations (200, 300, and 400 mg·m−3), group (a) and (b) showed higher performance than group (c) possibly due to the limitation of biological diffusion regime which may produce a exceed biomass accumulation. The results also indicated that a good performance of degrading ET achieved by employing bio-rickling filter, and about 70 s (superficial gas velocity of 8.74 × 10−3 m·s−1) was the best EBRT for the biotrickling filter.

3.4. Effect of Spray Density on RE

The spray density was another key parameter in the biofiltration desulfurization process [37]. The effect of spray density on RE was investigated under experimental condition of which the temperature was 20–25°C and EBRT was 60 s. There were also three groups studied under constant and variable gradient inlet concentration (shown in Figure 6). It could be seen that an upper trend of removal efficiencies in each group presented in Figure 6 was obtained with constantly increasing spray density. As Figure 6 shows, the ET REs of three groups (a), (b), and (c) had greatly improved when the spray density was less than 0.19 m3·m−2 h−1; correspondingly, stable tendency of REs of three groups was achieved when the spray density was greater than 0.19 m3·m−2 h−1 and the RE values were about 60%.

Figure 6: The effects of spray density on ethanethiol removal efficiency.

Higher RE in biotrickling filter was found by increasing spray density probably mainly due to the metabolism of microorganisms [2729]. The degradation process of ET through biological flora fixed on inorganic ceramic needs to obtain appropriate amount of nutrients from nutrient solution to maintain normal metabolism and growth of microorganisms [12]. So the amount of nutrients provided by nutrient solution became a limitation to the speed of metabolism of microorganisms. Thus increased spray density could stimulate the activity of microorganism metabolism because of the increase in the concentration of necessary nutrients [29]. When the spray density added up to a certain extent (0.24 m3·m−2 h−1), the moisture content of packing materials surface would increase excessively and then resulted in a growth formation of liquid membrane on microbial membrane [27]. However, intemperate growth and excessive thick liquid membrane could affect the ET gas molecules inside diffusion with a result that microorganisms could not degrade ET successively or well. On the other hand, due to both reasons of nutrient limitation and hardly insoluble properties in water of ET (Henry coefficient: 0.15–0.21) [1], the RE increased extremely little even if the spray density was added, generally, the RE hardly increased. And the optimum spray density of nutrient solution in this study was 0.24 m3·m−2 h−1.

3.5. Orthogonal Experiments

Three-factor and three-level orthogonal experiments were designed to decide the optimized condition of biotrickling filter. The main three investigated factors of orthogonal experiment were spray density (0.08, 0.24, and 0.39 m3·m−2 h−1), EBRT (34.34, 68.67, and 137.34 s), and inlet concentration (200, 300, and 400 mg·m−3). The experimental result was shown in Table 1. According to the range value of each factor, in present study situation, the affecting degree of each factor was spray density, inlet concentration, and EBRT. However, the optimized condition combined with previous experimental results could be determined as 0.24 m3·m−2 h−1 of spray density, less than 250 mg·m−3 of inlet concentration, and 68.7 s of the EBRT. However, the ethanethiol concentration after treatment by using the present biotrickling filter did not exceed 10.0 mg·L−1 which was the maximum allowable concentration in an environment in regulations in this condition [2]. Thus, this study method was satisfying and was applied to remove low concentration of ethanethiol. Besides, according to research by Huaxin et al. [31], the bottom layer removed more than 50% of the target waste gas when the inlet concentration was lower than 70 mg·m−3, but with the inlet concentration increasing to more than 90 mg·m−3, the middle and upper layer played a major role for removal and the contribution of the bottom decreased to 35%. Therefore, the higher RE could probably reach more than 66% by using multilayer filler with more than three layers.

Table 1: The result of orthogonal experiments.
3.6. pH Values and Metabolic Product Analysis

Because no biotransformation exists to consume sulfate which was the major metabolic products [30] of ET in biotrickling filter, it would accumulate in the nutrient solution and the pH would drop with an increase in operation time. The pH values and sulfate concentrations of nutrient solution for ET removal were regularly determined in biotrickling filter during 3-4-week operation period. And the results were shown in Figure 7.

Figure 7: (a) The effects of ethanethiol inlet loads on sulfate ion concentration and pH and (b) continuous operation conversion rate and ethyl mercaptan removal rate.

As Figure 7(a) shows, there was a linear relationship between ET various inlet loads and sulfate concentration and pH values of nutrient solution. When the ET various inlet loads (g·m−3 h−1) added from 7.39 to 11.34 g·m−3 h−1, the sulfate concentration ranged from 15.36 to 417.63 mg·L−1, and pH values of nutrient solution dropped from 7.7 to 4.9. Thus sulfate produced in the biofiltration desulfurization process was certain. After the values of pH added up to 5, there was no obvious change of trend of concentration even though various inlet loads added. That probably due to the weak alkaline ion besides such as and produced in desulfurization process [3]. There were some pale yellow substances with some characteristics of elemental sulfur material produced in the reactor, which makes the function of spray liquid did not further acidification [37]. In addition, the added concentrations of did not make a difference with pH values decreasing, indicating that the variation of pH nearly had no effect on the microbial degradation of ET in biotrickling filter.

Figure 7(b) was shown the relationship between removal efficiencies of ET and conversion of during the operation period. The conversion rates of showed lower trend at first with the maximum value of 99.62 mg·d−1 and the minimum value of 64.22 mg·d−1 under continuous operation in biotrickling filter reactor, and then there was a slight rise about 70–80 mg·d−1. Therefore the conversion rate could keep at a high level for a longtime-running regime in the bioreactor. An unobvious drop magnitude of ET removal efficiency from 64.98% to 62.30% could be also seen.

3.7. Kinetic Analysis

Biotrickling filter reactor was a kind of nonsubmerged gas phase bioreactor [38]. Based on the research hypothesis that the microorganism fixed on inorganic packing surface distributes evenly, the airstream mode was plug-flow and oxygen condition was not a limitation for biological aerobic respiration, consult relevant information literature [38, 39], Michaelis-Menien equation was an appropriate biological degradation mode for this studied desulfurization process:where (g·m−3 d−1) is substrate utilization rates, (g·m−3 d−1) is the maxim removal rates, and (mg·m−3) is concentration of contaminant and is half-saturation constant in equation. A simplified equation (7) obtains by linearizing the equation of Michaelis-Menien Mode through Lineweaver-Burk:where is removal rates of contaminant and is log-average concentration of contaminant . In addition, (mg·L−1) and (mg·L−1) are the inlet concentrations and the outlet concentrations of gas compounds, respectively. (m3·h−1) is gas volume flow of gas compounds of ET. There the intercept and slope could be calculated from a linear relationship between and by analyzing experimental data because they do represent and .

The experimental data of ET outlet concentration during constantly stable long-running regime in biotrickling filter system had been fitted with Michaelis-Menien model [39]. And the running conditions were at a temperature of 25°C, inlet gas flow of about 0.15 m3·h−1, and the filter volume of 6.36 × 10−3 m3. The fitted result was shown in Figure 8. The results showed that it could better meet the Michaelis-Menien models by using biotrickling filter for the removal of ET. The correlation coefficient was 0.975 that indicated a good correlation. Two kinetic parameters could be calculated as  mg m−3 and  g·m−3 h−1.

Figure 8: Fitting result of biotrickling filter system for ethanethiol removal.

4. Conclusions

The desulfurization process of sulfur odor ET by microorganisms fixed on iron oxide-based porous ceramsite (IPC) in biotrickling filter was investigated. The results showed that the optimum running conditions of ET degradation were at the inlet concentration of less than 250 mg·m−3, the spray density of 0.24 m3·m−2 h−1, and the EBRT of 68.7 s. And more than 64% of ET removal efficiency was achieved in that case. In addition, the main metabolic product was under continuous long-running regime in filter, and the concentration of increased with pH dropping due to the increase of inlet loads. But both ranges of and pH had no significant effect on ET removal performance. The fitted experimental correlation coefficient , and the kinetic degradation parameters  mg·m−3 and  g·m−3 h−1. The ET desulfurization process could better meet with the Michaelis-Menien model.

Conflict of Interests

The authors declare that there is no conflict of interests regarding the publication of this paper.


The authors thank the financial support from Jiangsu Key Laboratory of Atmospheric Environment Monitoring and Pollution Control of Nanjing University of Information Science and Technology, Jiangsu Province Innovation Platform for Superiority Subject of Environmental Science and Engineering (no. KHK1203), and the Science and Technology Project of Anhui Province of China (no. 12010402097) for supporting this study.


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