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Journal of Chemistry
Volume 2015 (2015), Article ID 430328, 9 pages
http://dx.doi.org/10.1155/2015/430328
Research Article

Benefits and Limitations of Lab-on-a-Chip Method over Reversed-Phase High-Performance Liquid Chromatography Method in Gluten Proteins Evaluation

1Institute of Food Technology, University of Novi Sad, Bulevar Cara Lazara 1, 21000 Novi Sad, Serbia
2Agricultural Institute Osijek, Južno Predgrađe 17, 31000 Osijek, Croatia
3Faculty of Food Technology, J.J. Strossmayer University of Osijek, Franje Kuhača 18, 31000 Osijek, Croatia
4Faculty of Natural Science, University of Kragujevac, Radoja Domanovića 12, 34000 Kragujevac, Serbia

Received 28 November 2014; Revised 28 January 2015; Accepted 5 February 2015

Academic Editor: Marcone A. L. de Oliveira

Copyright © 2015 Dragan Živančev et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

RP-HPLC (reversed-phase high-performance liquid chromatography) is widely used to determine the amounts of the different gluten protein types. However, this method is time-consuming, especially at early stages of wheat breeding, when large number of samples needs to be analyzed. On the other hand, LoaC (Lab-on-a-Chip) technique has the potential for a fast, reliable, and automatable analysis of proteins. In the present study, benefits and limitations of Lab-on-a-Chip method over RP-HPLC method in gluten proteins evaluation were explored in order to determine in which way LoaC method should be improved in order to make its results more compliant with the results of RP-HPLC method. Strong correlation () was found between numbers of HMW glutenin peaks determined by LoaC and RP-HPLC methods. Significant correlations () were obtained between percentages of HMW and LMW glutenin subunits calculated with regard to total HMW + LMW area. Even more significant correlation () was found when percentages of individual HMW areas were calculated with regard to total HMW. RP-HPLC method showed superiority in determination of gliadins since larger number and better resolution of gliadin peaks were obtained by this method.