Benefits and Limitations of Lab-on-a-Chip Method over Reversed-Phase High-Performance Liquid Chromatography Method in Gluten Proteins Evaluation

Table 1

Apparent sizes and quantification of individual HMW-GS subunits for all examined bread wheat cultivars by LoaC method and quantification of individual HMW-GS subunits by RP-HPLC.

Subunits	Molecular weight (kDa)		Relative amount (%)^a		Relative amount (%)^b
	Mean	CV (%)	Mean	CV (%)	Mean	CV (%)
2 & 5	230.9	0.55	5.32	6.25	—	—
2	230.9	1.00	3.51	37.74	10.29	7.03
5	229.6	2.10	3.34	41.91	10.71	18.84
5 & 2 & 9	—	—	—	—	14.43	1.71
5 & 9	—	—	—	—	10.16	1.06
2^*	212.6	3.08	1.93	64.82	1.47	25.43
1	212.2	1.11	1.21	32.43	1.75	49.85
7	178.0	2.41	3.92	59.37	9.32	15.38
17 + 18 (2)^c	160.6	2.03	0.99	27.98	—	—
10	144.4	2.24	2.71	47.14	2.99	37.07
8	134.8	1.36	0.50	44.77	2.16	26.31
9	131.7	1.60	2.47	51.37	3.99	27.32
12	130.0	0.00	0.79	16.75	3.86	26.25
12 & 9	128.0	0.52	5.51	27.80	—	—
17 + 18 (1)^c	124.8	2.83	1.67	14.66	—	—
17	—	—	—	—	10.80	1.77
18	—	—	—	—	4.67	4.53

Expressed as relative amount: percentage of individual HMW area to total LMW + HMW area.
^bExpressed as relative amount: percentage of individual HMW area to total LMW + HMW area.
^cSubunits 17 and 18 were clearly distinguished by LoaC method, whereas by SDS-PAGE method these two subunits were overlapped (Marchetti-Deschmann et al., 2011) [8]. Therefore it was not possible to determine molecular weight of each separate subunit by LoaC method and they will be labelled as 17 + 18 (1) and 17 + 18 (2).