Journal of Chemistry / 2015 / Article / Tab 2

Research Article

Benefits and Limitations of Lab-on-a-Chip Method over Reversed-Phase High-Performance Liquid Chromatography Method in Gluten Proteins Evaluation

Table 2

Apparent sizes and quantification of individual HMW-GS subunits of nine bread wheat cultivars grown in Croatia by LoaC method and quantification of individual HMW-GS subunits by RP-HPLC.

CultivarSubunitsMolecular weight (kDa)Relative amount (%)aRelative amount (%)b
MeanCV (%)MeanCV (%)MeanCV (%)

Soissons5228.11.432.5416.8211.640.00
2*221.00.194.1818.651.6816.64
7171.20.081.261.557.635.51
10139.90.253.294.751.9314.50
8133.30.530.2614.071.924.56

Felix5223.81.041.6819.9812.254.42
2*202.20.731.077.071.4211.31
7171.91.151.471.3310.251.18
10140.30.761.9518.561.951.03
8133.20.740.390.662.5311.12

Divana5226.81.873.1732.0611.216.49
2*205.51.651.213.592.1714.38
7174.70.892.098.6612.955.01
10142.40.892.303.133.887.37
9129.00.382.8830.376.561.19

Zlata5229.81.383.0730.436.775.34
2*208.02.070.962.911.0012.57
7176.41.482.8310.029.345.05
10143.91.871.9910.631.5821.91
9130.31.032.4522.733.559.31

Seka5226.40.941.7271.3110.802.49
1210.91.240.8510.641.170.00
7174.90.852.0923.069.312.89
10142.41.191.7134.372.0017.90
9135.40.630.7416.322.058.73

Vulkan5225.50.352.4966.876.823.06
7174.10.571.470.608.181.45
10142.61.141.4611.613.284.53
8135.00.630.5357.522.766.48

Renata5229.10.562.3019.5313.552.58
1212.61.101.0913.281.2513.99
7176.21.121.878.039.015.17
10143.31.831.178.032.236.98
8135.00.840.6549.251.449.43

Aida5224.22.563.0023.5910.823.92
2*209.31.620.8320.231.3024.38
17 + 18 (2)160.62.030.9927.98
10140.81.661.7810.742.3712.53
17 + 18 (1)124.82.831.6714.66
1710.801.77
184.674.53

Tihana2232.81.491.7548.769.792.72
1213.21.491.691.802.8317.32
7175.50.932.317.348.392.93
12 & 9127.20.677.0026.59
123.024.74
94.893.35

Expressed as relative amount: percentage of individual HMW area to total LMW + HMW area.
bExpressed as relative amount: percentage of individual HMW area to total LMW + HMW area.

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