Benefits and Limitations of Lab-on-a-Chip Method over Reversed-Phase High-Performance Liquid Chromatography Method in Gluten Proteins Evaluation
Table 2
Apparent sizes and quantification of individual HMW-GS subunits of nine bread wheat cultivars grown in Croatia by LoaC method and quantification of individual HMW-GS subunits by RP-HPLC.
Cultivar
Subunits
Molecular weight (kDa)
Relative amount (%)a
Relative amount (%)b
Mean
CV (%)
Mean
CV (%)
Mean
CV (%)
Soissons
5
228.1
1.43
2.54
16.82
11.64
0.00
2*
221.0
0.19
4.18
18.65
1.68
16.64
7
171.2
0.08
1.26
1.55
7.63
5.51
10
139.9
0.25
3.29
4.75
1.93
14.50
8
133.3
0.53
0.26
14.07
1.92
4.56
Felix
5
223.8
1.04
1.68
19.98
12.25
4.42
2*
202.2
0.73
1.07
7.07
1.42
11.31
7
171.9
1.15
1.47
1.33
10.25
1.18
10
140.3
0.76
1.95
18.56
1.95
1.03
8
133.2
0.74
0.39
0.66
2.53
11.12
Divana
5
226.8
1.87
3.17
32.06
11.21
6.49
2*
205.5
1.65
1.21
3.59
2.17
14.38
7
174.7
0.89
2.09
8.66
12.95
5.01
10
142.4
0.89
2.30
3.13
3.88
7.37
9
129.0
0.38
2.88
30.37
6.56
1.19
Zlata
5
229.8
1.38
3.07
30.43
6.77
5.34
2*
208.0
2.07
0.96
2.91
1.00
12.57
7
176.4
1.48
2.83
10.02
9.34
5.05
10
143.9
1.87
1.99
10.63
1.58
21.91
9
130.3
1.03
2.45
22.73
3.55
9.31
Seka
5
226.4
0.94
1.72
71.31
10.80
2.49
1
210.9
1.24
0.85
10.64
1.17
0.00
7
174.9
0.85
2.09
23.06
9.31
2.89
10
142.4
1.19
1.71
34.37
2.00
17.90
9
135.4
0.63
0.74
16.32
2.05
8.73
Vulkan
5
225.5
0.35
2.49
66.87
6.82
3.06
7
174.1
0.57
1.47
0.60
8.18
1.45
10
142.6
1.14
1.46
11.61
3.28
4.53
8
135.0
0.63
0.53
57.52
2.76
6.48
Renata
5
229.1
0.56
2.30
19.53
13.55
2.58
1
212.6
1.10
1.09
13.28
1.25
13.99
7
176.2
1.12
1.87
8.03
9.01
5.17
10
143.3
1.83
1.17
8.03
2.23
6.98
8
135.0
0.84
0.65
49.25
1.44
9.43
Aida
5
224.2
2.56
3.00
23.59
10.82
3.92
2*
209.3
1.62
0.83
20.23
1.30
24.38
17 + 18 (2)
160.6
2.03
0.99
27.98
—
—
10
140.8
1.66
1.78
10.74
2.37
12.53
17 + 18 (1)
124.8
2.83
1.67
14.66
—
—
17
—
—
—
—
10.80
1.77
18
—
—
—
—
4.67
4.53
Tihana
2
232.8
1.49
1.75
48.76
9.79
2.72
1
213.2
1.49
1.69
1.80
2.83
17.32
7
175.5
0.93
2.31
7.34
8.39
2.93
12 & 9
127.2
0.67
7.00
26.59
—
—
12
—
—
—
—
3.02
4.74
9
—
—
—
—
4.89
3.35
Expressed as relative amount: percentage of individual HMW area to total LMW + HMW area.
bExpressed as relative amount: percentage of individual HMW area to total LMW + HMW area.
