Benefits and Limitations of Lab-on-a-Chip Method over Reversed-Phase High-Performance Liquid Chromatography Method in Gluten Proteins Evaluation
Table 3
Apparent sizes and quantification of individual HMW-GS subunits of nine bread wheat cultivars grown in Serbia by LoaC method and quantification of individual HMW-GS subunits by RP-HPLC.
Cultivar
Subunits
Molecular weight (kDa)
Relative amount (%)a
Relative amount (%)b
Mean
CV (%)
Mean
CV (%)
Mean
CV (%)
Etida
5
237.8
0.15
3.53
30.28
9.72
0.20
7
184.0
0.46
4.30
35.62
10.49
3.12
10
148.1
0.62
2.70
23.92
3.62
7.44
9
133.6
1.16
2.38
22.13
3.37
1.14
Ljiljana
5
233.4
1.27
4.58
20.94
—
—
5 & 9
—
—
—
—
10.16
1.06
7
183.8
1.23
6.50
13.02
9.91
0.73
10
147.8
0.67
4.52
9.30
5.27
1.02
9
131.1
0.70
1.12
14.79
—
—
Pobeda
5
232.1
0.79
5.68
9.24
11.79
2.66
2*
216.3
0.26
3.35
24.39
1.65
0.00
7
181.3
0.35
7.21
34.01
9.91
1.13
10
147.0
0.29
4.34
26.86
4.28
10.48
9
130.9
0.59
4.27
40.61
4.06
18.78
Bastijana
5
234.6
1.27
5.04
8.97
11.91
0.50
2*
216.4
0.39
1.07
66.38
1.65
1.21
7
182.5
0.08
7.51
19.71
8.74
2.74
10
147.9
0.29
4.89
12.12
2.27
7.91
9
131.8
0.16
3.62
16.12
3.62
0.55
NS3-5299
5
233.5
1.91
4.58
9.12
11.25
0.36
2*
218.5
0.29
1.75
18.35
1.25
3.68
7
182.7
1.47
4.18
34.82
7.67
1.05
10
147.8
0.57
1.84
0.10
4.15
0.97
9
131.5
1.61
2.32
24.33
3.95
1.02
Simonida
2
229.1
0.65
4.24
2.90
9.50
1.33
7
177.9
0.32
3.04
11.50
6.69
1.35
12 & 9
128.3
0.17
3.96
16.46
—
—
9
—
—
—
—
3.95
0.91
12
—
—
—
—
5.24
3.10
Dragana
2
229.8
0.46
3.15
8.06
10.75
1.54
7
177.0
0.28
6.59
1.13
10.04
2.88
12 & 9
127.9
0.33
6.25
6.39
—
—
9
—
—
—
—
4.25
0.49
12
—
—
—
—
4.20
3.93
Zvezdana
2
232.2
0.70
4.91
3.88
11.11
1.38
7
179.1
0.20
5.17
2.60
9.42
0.61
12 & 9
128.6
0.06
4.83
21.54
9
—
—
—
—
3.63
4.22
12
—
—
—
—
2.95
1.30
Nevesinjska
5 & 2
230.9
0.55
5.32
6.25
—
—
5 & 2 & 8
—
—
—
—
14.43
1.71
2*
216.5
0.36
2.96
11.42
1.11
3.72
7
183.4
0.04
6.73
17.34
10.52
1.76
10
147.0
0.43
4.05
6.18
3.04
0.68
8
137.7
0.21
0.67
9.24
—
—
12
130.0
0.00
0.79
16.75
—
—
Expressed as relative amount: percentage of individual HMW area to total LMW + HMW area.
bExpressed as relative amount: percentage of individual HMW area to total LMW + HMW area.
