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Journal of Chemistry
Volume 2015, Article ID 530607, 6 pages
http://dx.doi.org/10.1155/2015/530607
Research Article

Cartilage Tissue Engineering Using Combination of Chitosan Hydrogel and Mesenchymal Stem Cells

1Institute of Tissue Engineering and Stem Cells, Nanchong Central Hospital and the Second Clinical Institute of North Sichuan Medical University, 97 Renmin South Road, Nanchong, Sichuan 637000, China
2Sichuan Medical University, 319 3rd Block of Zhongshan Road, Luzhou, Sichuan 646000, China
3School of Chemistry, University of Edinburgh, King’s Buildings, West Mains Road, Edinburgh EH9 3JF, UK

Received 2 October 2015; Accepted 26 October 2015

Academic Editor: Jin Geng

Copyright © 2015 Dechao Yuan et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

A novel chitosan hydrogel with high porosity was fabricated by a crosslinking method. Cartilage tissue engineering formed after mesenchymal stem cells was cultured on this hydrogel scaffold for 12 weeks. The immunohistochemistry tests demonstrated that the obtained cartilage had the specific histological properties of natural cartilage. And the qPCR tests also proved that the genes for type II collagen in the obtained cartilage were expressed the same as in the natural one.