Extraction Algae Installation Temp. [°C] Pressure [bar] Extract Extraction yield Reference SFE with CO2 Botryococcus braunii, Chlorella vulgaris, Dunaliella salina, and Arthrospira maxima , whole, crushed, and slightly crushedFlow 40–60 125, 200, and 300 B. braunii : alkadienes;C. vulgaris : carotenoids (canthaxanthin, astaxanthin);D. salina : β -carotene (trans- and cis-isomer); A. maxima : GLA, C18:3 ω 6 (CO2 and CO2 + 10 mol% ethanol) and lipidsTotal GLA: 45%: 35.0 MPa, 333.1 K with the mixture (CO2 + 10 mol% ethanol) [26 ] SFE with CO2 and ethanol (9.4% mass) Haematococcus pluvialis and Arthrospira maxima (Spirulina )Column; flow rate: 1 mL/min 60 300 H. pluvialis : astaxanthin, A. maxima : phycocyaninAstaxanthin: 1.7% mass (no effect of ethanol); phycocyanin: 1.1% mass (CO2 ), 1.7% mass (CO2 + ethanol) [2 ] SFE with CO2 and ethanol (9.23 mL/g) Haematococcus pluvialis Biomass 6.5 g; CO2 flow rate: 6.0 mL/min; time: 20 min 50 310 Pigment (astaxanthin) Astaxanthin: 74% (11 mg/g dry cells), 8 extraction cycles [5 ] SFE with CO2 Chlorella vulgaris Flow type 40, 55 350 Carotenoids, lipids Carotenoids and lipids: improved for crushed cells and at higher p [25 ] SFE with CO2 Botryococcus braunii Flow type 50–85 200–250 Fatty acids Lipid yield decreased with temperature and increased with pressure [36 ] SFE with CO2 Botryococcus braunii and Chlorella vulgaris Flow type 40 300 (B. braunii ) and 350 (C. vulgaris ) B. braunii : hydrocarbonsC. vulgaris : carotenoids (canthaxanthin and astaxanthin) The extraction yield of carotenoids increased with the degree of crushing of the microalga [37 ] SFE with CO2 Arthrospira platensis (Spirulina )Time: 4 h 48 200 85 g/kg of flavonoids; 78 g/kg of β -carotene; 113 g/kg of vitamin A; 3.4 g/kg of α -tocopherol; fatty acids: palmitic (35%), linolenic (22%), and linoleic (21%) Yield of the extracts from S. platensis –10 g/kg [38 ] SFE with CO2 (soybean oil and ethanol as modifier) Chlorella vulgaris Flow type 40 300 Carotenoids: 69%, crushing strongly improved extraction recovery [39 ] SFE with CO2 Haematococcus pluvialis Time: 4 h 70 500 Astaxanthin The predicted amount of astaxanthin extracted was 23 mg/g [40 ] SFE with CO2 and ethanol (0.856 mL/g of biomass) Arthrospira platensis (Spirulina )Time: 1 h 40 400 -linolenic acidA recovery of 102% GLA [41 ] SFE with CO2 and ethanol Arthrospira maxima (freeze-dried)Flow type 50–60 250 γ -linolenic acid and lipidsGLA and lipids: up to 45% [27 , 28 ] SFE with CO2 Synechococcus sp.Flow type 50 (for carotenoids) and 60 (for chlorophyll) 300 (for carotenoids) and 500 (for chlorophyll) Carotenoids and chlorophylls Carotenoids (1.5 μ g/mg dry weight of microalga); chlorophyll a (0.71 μ g/mg dry weight of microalga) [42 ] SFE with CO2 and CO2 : ethanol Arthrospira platensis (Spirulina )Pilot-scale plant 75 (CO2 ) 55 and (CO2 : EtOH) 320 (CO2 ) and 78.6 (CO2 : EtOH) Vitamin E CO2 : yield 0.85%; 16 mg of vitamin E/g of extract; CO2 : ethanol: yield 8.1%; 0.49 mg of vitamin E/g of extract; CO2 (w/w) 69% [43 ] PLE using mixture of ethanol : ethyl lactate Arthrospira platensis (Spirulina )Time: 15 min 180 207 -linolenic acidTotal yields up to 21% (w/w), for a solvent composition of ethanol : ethyl lactate (50 : 50, v/v), GLA recovery of 68% [44 ]