Storage Effect on Phenols and on the Antioxidant Activity of Extracts from Anemopsis californica and Inhibition of Elastase Enzyme

Table 3

Changes in total flavonoids during storage of extracts from Anemopsis californica.

Part of the plant	Treatment		Storage time (days
	(°C)		0	30	60	90	120	180
Leaf	50	PL	15.8 ± 1.2	12.8 ± 2.0	10.3 ± 1.1	08.4 ± 0.9	08.4 ± 1.7	06.6 ± 0.4^b
		NP	15.8 ± 1.2	12.0 ± 1.4	10.9 ± 2.9	07.7 ± 1.0	06.5 ± 0.7	05.2 ± 0.2^a
	25	PL	15.8 ± 1.2	14.5 ± 0.3	14.4 ± 0.5	12.2 ± 0.2	09.6 ± 0.9	08.2 ± 0.1^d
		NP	15.8 ± 1.2	13.3 ± 0.1	12.5 ± 0.4	10.3 ± 0.1	08.7 ± 0.7	07.4 ± 0.4^c
	4	PL	15.8 ± 1.2	15.5 ± 0.8	15.3 ± 0.6	13.0 ± 0.2	11.5 ± 0.2	10.4 ± 0.7^e
		NP	15.8 ± 1.2	15.0 ± 1.9	14.2 ± 2.1	11.2 ± 0.1	10.9 ± 0.9	08.7 ± 0.2^d
	−20	PL	15.8 ± 1.2	15.5 ± 1.5	15.3 ± 1.1	14.1 ± 0.8	12.3 ± 0.7	11.4 ± 0.4^f
		NP	15.8 ± 1.2	15.2 ± 0.2	13.9 ± 0.6	12.2 ± 0.2	11.1 ± 0.9	10.7 ± 0.3^e
Stem	50	PL	23.7 ± 3.1	19.5 ± 2.1	17.3 ± 1.3	16.4 ± 1.5	13.3 ± 0.8	10.7 ± 0.1^c
		NP	23.7 ± 3.1	18.3 ± 0.9	16.6 ± 1.2	14.5 ± 1.7	13.0 ± 0.7	08.8 ± 0.3^a
	25	PL	23.7 ± 3.1	22.1 ± 0.4	19.2 ± 0.7	18.7 ± 1.9	14.9 ± 1.2	13.1 ± 1.1^d
		NP	23.7 ± 3.1	21.3 ± 2.2	18.3 ± 2.0	16.4 ± 1.1	13.8 ± 0.9	09.7 ± 0.8^b
	4	PL	23.7 ± 3.1	23.2 ± 1.4	23.2 ± 1.3	19.7 ± 1.3	18.0 ± 1.1	15.2 ± 1.7^e
		NP	23.7 ± 3.1	23.0 ± 1.9	21.1 ± 2.3	18.5 ± 2.1	15.7 ± 0.9	13.8 ± 1.1^d
	−20	PL	23.7 ± 3.1	23.5 ± 1.8	23.2 ± 1.7	20.6 ± 0.7	18.0 ± 0.3	16.6 ± 1.0^f
		NP	23.7 ± 3.1	23.0 ± 0.4	23.0 ± 2.1	19.5 ± 2.2	16.8 ± 0.7	15.4 ± 0.6^e
Rhizome	50	PL	38.1 ± 1.9	32.4 ± 1.8	28.6 ± 2.9	25.1 ± 3.0	21.0 ± 2.4	17.5 ± 1.0^b
		NP	38.1 ± 1.9	29.7 ± 0.9	26.3 ± 1.2	19.4 ± 2.1	16.4 ± 0.9	13.3 ± 0.7^a
	25	PL	38.1 ± 1.9	36.2 ± 1.1	31.6 ± 2.5	29.0 ± 2.3	24.8 ± 2.1	20.6 ± 1.8^c
		NP	38.1 ± 1.9	35.1 ± 1.8	30.9 ± 1.5	27.4 ± 1.1	23.2 ± 1.0	17.1 ± 1.7^b
	4	PL	38.1 ± 1.9	37.3 ± 2.1	37.0 ± 2.2	30.9 ± 3.0	29.3 ± 2.2	24.4 ± 2.1^d
		NP	38.1 ± 1.9	37.0 ± 0.8	33.5 ± 1.4	28.2 ± 1.6	25.9 ± 0.6	21.7 ± 2.2^c
	−20	PL	38.1 ± 1.9	37.7 ± 1.1	37.3 ± 1.2	32.4 ± 0.7	30.1 ± 0.9	27.8 ± 1.2^e
		NP	38.1 ± 1.9	37.7 ± 1.3	37.0 ± 2.4	32.0 ± 1.5	29.3 ± 3.1	24.4 ± 2.3^d

Data are reported as mg of quercetin equivalents (QE)/g of dry weight. PL: protected from light using amber bottle; NP: not using amber bottle. Mean ± standard deviation (SD) of three independent experiments. Different letters in each line indicate significant difference at .