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Journal of Chemistry
Volume 2015, Article ID 648361, 6 pages
http://dx.doi.org/10.1155/2015/648361
Research Article

Analysis of Bovine Serum Albumin Ligands from Puerariae flos Using Ultrafiltration Combined with HPLC-MS

1College of Chemistry and Chemical Engineering, Central South University, Changsha, Hunan 410083, China
2School of Environmental Science and Engineering, Hubei Polytechnic University, Hubei Key Laboratory of Mine Environmental Pollution Control and Remediation, Huangshi, Hubei 435003, China
3Institute of Bast Fiber Crops, Chinese Academy of Agricultural Sciences, Changsha 410205, China

Received 24 September 2015; Revised 26 November 2015; Accepted 30 November 2015

Academic Editor: Eulogio J. Llorent-Martinez

Copyright © 2015 Ping Tang et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

Rapid screening techniques for identification of active compounds from natural products are important not only for clarification of the therapeutic material basis, but also for supplying suitable chemical markers for quality control. In the present study, ultrafiltration combined with high performance liquid chromatography-mass spectrometry (HPLC-MS) was developed and conducted to screen and identify bovine serum albumin (BSA) bound ligands from Puerariae flos. Fundamental parameters affecting the screening like incubation time, BSA concentration, pH, and temperature were studied and optimized. Under the optimum conditions, nine active compounds were identified by UV and MS data. The results indicated that this method was able to screen and identify BSA bound ligands form natural products without the need of preparative isolation techniques. Moreover, the method has more effective with easier operation procedures.