Analysis of Bovine Serum Albumin Ligands from Puerariae flos Using Ultrafiltration Combined with HPLC-MS

Table 2

Comparison of ultrafiltration and conventional bioassay method in analysis of active compounds.

Item	Ultrafiltration	Conventional bioassay [3]
Theory	Protein-drug binding; Membrane separation	Solvent extraction; Chromatographic separation
Procedure	Incubation of mixture; Ultrafiltration; HPLC-MS analysis	Solvent extraction; Repeated fractionation and isolation; Structural characterization; Pharmacological test
Time	About three hours	Several days
Reusable	The devices could be reused less than 5 times	The recycle of reagents needs much time and energy
Temperature	Room temperature	Room temperature