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Journal of Chemistry
Volume 2015, Article ID 784626, 10 pages
http://dx.doi.org/10.1155/2015/784626
Research Article

Effects of Self-Assembled Monolayers with Different Chemical Groups on Ovarian Cancer Cell Line Behavior In Vitro

1Department of Obstetrics and Gynecology, Guangzhou General Hospital of Guangzhou Military Command, Guangzhou 510010, China
2Guangzhou University of Chinese Medicine, Guangzhou 510010, China
3Panyu Maternal and Child Care Service Centre of Guangzhou, Guangzhou 511400, China
4Hospital of Orthopedics, Guangzhou General Hospital of Guangzhou Military Command, Guangzhou 510010, China

Received 10 May 2015; Revised 22 July 2015; Accepted 26 July 2015

Academic Editor: Tanaji Talele

Copyright © 2015 Jie Li et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

In addition to serving as a physical support, the extracellular matrix (ECM) actively influences cell behavior. However, the definitive effects of different chemical structures present in the ECM on cell behavior remain obscure. The current study aimed to investigate the effects of different chemical structures present in the ECM on cellular physiology using the ovarian cancer cell line SKOV-3 as a model. Self-assembled monolayers (SAMs) with different chemical modifications, including methyl (-CH3), hydroxyl (-OH), amino (-NH2), carboxyl (-COOH), and mercapto (-SH) groups, were used as microenvironmental models to explore the effects of different structures on SKOV-3 cells. The cell morphology, cell adhesion, cytotoxicity, and functional alterations in cancer cells cultured on different SAMs were analyzed. The results showed that SKOV-3 cells cultured on -NH2 surfaces exhibited the largest contact area, whereas those on -CH3 surfaces exhibited the smallest contact area and mostly rounded morphologies. Additionally, -NH2 and -COOH promoted cell proliferation and adhesion, whereas CH3 inhibited adhesion, leading to G1 arrest during the cell cycle and resulting in cell apoptosis. This study may provide useful information for reconstruction of the ECM and for controlling cell behavior in related areas of study.