Development and Validation of a Simple and Selective Analytical HPLC Method for the Quantification of Oxaliplatin

Table 1

Variation in analytical method conditions. OXPt samples (15.0 µg/mL) were analyzed in HPLC equipment using a C₁₈ column, with UV detection fixed at 255 nm, at 25°C.

Mobile phase (volume : volume)	Injection volume (µL)	Flow rate (mL/min)	Result
Acetonitrile : water (90 : 10)	50	1	Peak tailing
Acetonitrile : water (80 : 20)	50	1	Peak tailing
Acetonitrile : water (70 : 30)	50	1	Peak tailing
Acetonitrile : water (50 : 50)	50	1	Peak tailing
Acetonitrile : water (20 : 80)	50	1	Peak tailing
Acetonitrile : water : Methanol (60 : 30 : 10)	50	1	Peak spike
Methanol : water (85 : 15)	50	1	Early peak
Methanol : water (65 : 35)	50	1	Early peak
Methanol : water (70 : 30)	10	0.5	Peak tailing
Methanol : water (70 : 30)	30	0.3	Broad peak
Methanol : water (30 : 70)	50	1	Broad peak
Acid solution : acetonitrile (80 : 20)	20	1	Early peak
Acid solution : acetonitrile (90 : 10)	20	1	Early peak
Acid solution : acetonitrile (95 : 5)	20	1	Suitable peak