Enzymatic Transformation and Bonding of Sulfonamide Antibiotics to Model Humic Substances
Table 4
Percentage of sulfonamides recovered through sequential extraction after 15 h of enzymatic transformation with laccase from Trametes versicolor in the absence (control) and presence of substituted phenols (standard deviation in parentheses).
Sum of all fractions = recovery
Reaction solution = unreacted
Buffer pH 1.7 = easily desorbable
NaOH/methanol = total desorbable
DMSO = sequestered
SAA
Control
98 (1)
98 (1)
n.d.
n.d.
n.d.
Catechol
84 (11)
n.d.
26 (10)
43 (13)
15 (13)
Guaiacol
58 (8)
n.d.
4 (1)
53 (8)
1 (1)
Vanillin
21 (7)
n.d.
8 (1)
10 (7)
3 (1)
SPY
Control
99 (0.1)
99 (0.1)
n.d.
n.d.
n.d.
Catechol
49 (10)
n.d.
n.d.
39 (10)
10 (2)
Guaiacol
33 (4)
n.d.
1 (0)
31 (4)
1 (0)
Vanillin
27 (4)
n.d.
6 (2)
17 (1)
4 (1)
SDT
Control
98 (1)
98 (1)
n.d.
n.d.
n.d.
Catechol
94 (7)
n.d.
48 (23)
45 (20)
1 (1)
Guaiacol
66 (7)
n.d.
15 (2)
18 (2)
34 (6)
Vanillin
92 (3)
11 (2)
23 (9)
24 (4)
35 (11)
n.d.: not determined at a detection limit of 4 mol mL−1.
