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Journal of Chemistry
Volume 2015, Article ID 836076, 9 pages
Research Article

Layer-by-Layer Assembly of Polysaccharides and 6,10-Ionene for Separation of Nitrogen-Containing Pharmaceuticals and Their Enantiorecognition by Capillary Electrophoresis

Analytical Chemistry Division, Department of Chemistry, Lomonosov Moscow State University, Leninskie Gory 1/3, Moscow 119991, Russia

Received 11 July 2014; Revised 14 October 2014; Accepted 14 October 2014

Academic Editor: Hasan Uslu

Copyright © 2015 Anna Ioutsi et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.


Two silica capillaries modified layer-by-layer with 6,10-ionene and N-(3-sulfo-3-carboxy)-propionylchitosan (SCPC) and with 6,10-ionene and dextran sulfate (DS) were prepared and investigated. Dynamic coating of the capillary efficiently reduces the adsorption of the background electrolyte, sample matrix components, and analytes on its inner wall. Such coatings effect good reproducibility and sensitivity of determination. We demonstrate that separation of betablockers, calcium channel blockers, alpha-adrenergic agonists, H1-blockers, and diuretics was the most efficient and rapid separation with a capillary modified with dextran sulfate. Tetrahydrozoline, carbinoxamine, and furacilin, which are commonly employed as treatments for allergic rhinitis, were identified in human urea. Their concentrations, independently verified by HPLC, were found to be , , and μg mL−1, with , 0.03, 0.10 μg mL−1, and , 0.8, 0.6 μg mL−1, respectively.