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Journal of Chemistry
Volume 2015, Article ID 931908, 5 pages
http://dx.doi.org/10.1155/2015/931908
Research Article

Stereoselective Separation and Acute Toxicity of Tau-Fluvalinate to Zebrafish

Qi Jia,1,2 Nana Xu,1,2 Pengqian Mu,1,2 Bo Wang,1,2 Shuming Yang,1,2 and Jing Qiu1,2

1Key Laboratory of Agro-Product Quality and Safety, Institute of Quality Standard and Testing Technology for Agro-Products, Chinese Academy of Agricultural Sciences, Beijing 100081, China
2Key Laboratory of Agri-Food Quality and Safety, Ministry of Agriculture, Beijing 100081, China

Received 13 March 2015; Revised 21 May 2015; Accepted 26 May 2015

Academic Editor: Qing X. Li

Copyright © 2015 Qi Jia et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

Tau-fluvalinate (TFLV) is one of the most potent chiral synthetic pyrethroids to control a wide range of pests in agricultural fields, especially in apiary. In this study, two stereoisomers of TFLV were fully separated by high-performance liquid chromatography (HPLC) with a semipreparative chiral column using cellulose-tris(3,5-dimethylphenylcarbamate) as chiral stationary phase and n-hexane and 2-propanol (96/4, v/v) as mobile phase at a flow rate of 2.5 mL min−1. The (+)-stereoisomer was first eluted by detecting with an optical rotation detector. After obtaining pure single stereoisomer of TFLV, acute toxicities of each isomer and TFLV standard to zebrafish were evaluated. The results showed that the (+)-stereoisomer exhibited 273.4 times higher toxicity than the (−)-stereoisomer and 6.7 times higher than TFLV standard, according to their LC50 values at 96 h after exposure. This indicates that the toxicity of TFLV mainly originates from (+)-stereoisomer. These results are very helpful to prepare single stereoisomer of chiral pesticides and evaluate their different toxicological effects to aquatic organisms.