Research Article
Streptomyces lavendulae Protease Inhibitor: Purification, Gene Overexpression, and 3-Dimensional Structure
Table 1
Bacterial strains and plasmids used in this study.
| | Strain/plasmid | Description | Source |
| | Strains: E. coil JM109 | F′ traD36 proA+B+ lacI Δ(lacZ)M15/Δ(lac-proAB) glnV44 e14− gyrA96 recA1 relA1 endA1 thi hsdR17 | New England Biolabs |
| | E. coil DA100 | JM109 (pDA1) | Competent cells |
| | Plasmids: pACYC177/ET3d/yNMT | Promoter for expression: T7 (phi10)
Replicon: p15A | ATCC |
| | pDA1 | pACYC177/ET3d/yNMT + 1626 bp sti1 gene | This work |
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