Research Article
Monitoring Antibiotic Residues and Corresponding Antibiotic Resistance Genes in an Agroecosystem
Table 3
Polymerase chain-reaction (PCR) primers for tetracycline- (TC-) and sulfonamide- (SA-) resistant gene classes.
| Gene | Primer | Sequences | Annealing temp. (°C) | Amplicon size (bp) |
| tet(S) | tetS-FW | GAAAGCTTACTATACAGTAGC | 50 | 169 | tetS-RV | AGGAGTATCTACAATATTTAC |
| tet(T) | tetT-FW | AAGGTTTATTATATAAAAGTG | 46 | 169 | tetT-RV | AGGTGTATCTATGATATTTAC |
| otr(A) | otrA-FW | GGCATYCTGGCCCACGT | 66 | 212 | otrA-RV | CCCGGGGTGTCGTASAGG |
| sul(I) | sulI-FW | CGCACCGGAAACATCGCTGCAC | 55.9 | 163 | sulI-RV | TGAAGTTCCGCCGCAAGGCTCG |
| sul(II) | sulII-FW | TCCGGTGGAGGCCGGTATCTGG | 60.8 | 191 | sulII-RV | CGGGAATGCCATCTGCCTTGAG |
| sul(III) | sulIII-FW | TCCGTTCAGCGAATTGGTGCAG | 60 | 128 | sulIII-RV | TTCGTTCACGCCTTACACCAGC |
| sul(A) | sulA-FW | TCTTGAGCAAGCACTCCAGCAG | 60 | 229 | sulA-RV | TCCAGCCTTAGCAACCACATGG |
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Forward. Reverse.
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