Journal of Chemistry / 2015 / Article / Tab 3

Research Article

Monitoring Antibiotic Residues and Corresponding Antibiotic Resistance Genes in an Agroecosystem

Table 3

Polymerase chain-reaction (PCR) primers for tetracycline- (TC-) and sulfonamide- (SA-) resistant gene classes.

GenePrimerSequencesAnnealing temp. (°C)Amplicon size (bp)

tet(S)tetS-FWGAAAGCTTACTATACAGTAGC50169
tetS-RVAGGAGTATCTACAATATTTAC

tet(T)tetT-FWAAGGTTTATTATATAAAAGTG46169
tetT-RVAGGTGTATCTATGATATTTAC

otr(A)otrA-FWGGCATYCTGGCCCACGT66212
otrA-RVCCCGGGGTGTCGTASAGG

sul(I)sulI-FWCGCACCGGAAACATCGCTGCAC55.9163
sulI-RVTGAAGTTCCGCCGCAAGGCTCG

sul(II)sulII-FWTCCGGTGGAGGCCGGTATCTGG60.8191
sulII-RVCGGGAATGCCATCTGCCTTGAG

sul(III)sulIII-FWTCCGTTCAGCGAATTGGTGCAG60128
sulIII-RVTTCGTTCACGCCTTACACCAGC

sul(A)sulA-FWTCTTGAGCAAGCACTCCAGCAG60229
sulA-RVTCCAGCCTTAGCAACCACATGG

Forward.
Reverse.