Research Article

Development of Au-Nanoprobes Combined with Loop-Mediated Isothermal Amplification for Detection of Isoniazid Resistance in Mycobacterium tuberculosis

Figure 2

Determining the optimal temperature for LAMP. Lanes 1 and 14 (M): Ladder DNA marker; Lanes 2–13: reaction for 60 min at 59, 61, 63, and 65°C; Mut as MDR-TB; STB as H37RV; Neg as negative. All LAMP products were electrophoresed on 2% agarose gels and stained with MaestroSafe Nucleic Acid Gel Stain Sample (Maestrogen).