Development of Au-Nanoprobes Combined with Loop-Mediated Isothermal Amplification for Detection of Isoniazid Resistance in Mycobacterium tuberculosis

Figure 3

Determining the DNA templates for LAMP of MDR-TB members via the Au-nanoprobes assay. (a) Agarose gel showed LAMP products. Lane 1 (M): Ladder DNA marker; Lanes 1–12: DNA templates for LAMP at 10⁰–10⁻⁵, Mut as MDR-TB, STB as H37RV, and Neg as negative. All LAMP products were electrophoresed on 2% agarose gels and stained with MaestroSafe Nucleic Acid Gel Stain Sample (Maestrogen). (b) Corresponding results recorded visually after salt challenge. Red colour showed the presence of the target and purple indicated its absence.

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