A Benzochalcone Derivative, (E)-1-(2-hydroxy-6-methoxyphenyl)-3-(naphthalen-2-yl)prop-2-en-1-one (DK-512), Inhibits Tumor Invasion through Inhibition of the TNFα-Induced NF-κB/MMP-9 Axis in MDA-MB-231 Breast Cancer Cells

Figure 3

Effect of DK-512 on the inhibition of TNFα-induced MMP-9 mRNA expression. (a) RT-PCR analysis. MDA-MB-231 cells were treated with 10 ng/mL TNFα for 12 and 18 h. Total RNA was isolated and RT-PCR was performed. GAPDH mRNA was used as an internal control. Band intensities were measured using ImageJ software. The data shown represent the mean ± SD (). . P value was analyzed by Sidak’s test. (b) RT-PCR analysis. MDA-MB-231 cells were pretreated with 5 μM DK-512 for 30 min before the addition of 10 ng/mL TNFα. After 12 h, total RNA was isolated and RT-PCR was performed. GAPDH mRNA was used as an internal control. Band intensities were measured using ImageJ software. The data shown represent the mean ± SD (). . value was analyzed by Sidak’s test. (c) Real-time PCR. MDA-MB-231 cells were pretreated with different concentrations of DK-512 before the addition of 10 ng/mL TNFα. After 12 h, total RNA was isolated and MMP-9 mRNA levels were measured by quantitative real-time PCR. The relative fold changes were normalized to GAPDH mRNA in the same sample. The data shown represent the mean ± SD (). ns: not significant compared to TNFα-only treatment. . P value was analyzed by Sidak’s test.

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