Rapid Determination of the Monosaccharide Composition and Contents in Tea Polysaccharides from Yingshuang Green Tea by Pre-Column Derivatization HPLC

Table 1

Retention time, regression analysis, and LOD for the pre-column PMP derivatization HPLC method.

Carbohydrates	Time (min)	Regression equation,		Correlation coefficient	Detection limit (μM)
		Mannose	6.673			−0.0496	2593.1	0.9994	0.09
Ribose	7.292	−0.0455	2268.8	0.9994	0.10
Rhamnose	8.244	−0.0287	1875.1	0.9993	0.19
Glucuronic acid	10.771	−0.0957	1997.6	0.9991	0.30
Galacturonic acid	11.672	−0.1579	2174.9	0.9983	0.20
Glucose	12.478	+0.0139	2097.8	0.9991	0.10
Xylose	13.678	−0.0259	2577.2	0.9980	0.07
Galactose	14.386	+0.0153	2480.6	0.9994	0.09
Arabinose	15.12	+0.0211	2436.6	0.9994	0.10

a: and are the peak area ratio of the analytes to the internal standard (fucose) and concentration of the analytes (0.2–2 μM), respectively.
b: LOD was defined as the value equal to a signal-to-noise ratio of three.