Table 3: Effect of alkaline solution concentration and temperatures on the fatty acid compositions of ZSKO.

Fatty acids (g/100 g)10% NaOH treatment for 1 hr14% NaOH treatment for 1 hr18% NaOH treatment for 1 hr20% NaOH treatment for 1 hr
20°C50°C80°CBoiling20°C50°C80°CBoiling20°C50°C80°CBoiling20°C50°C80°CBoiling

C14:1, 9tNDNDNDNDNDNDNDNDNDNDNDNDNDNDNDND
C14:1, 9cNDNDNDNDNDNDNDNDNDNDNDNDNDNDNDND
C16:07.23 0.047.14 0.086.88 0.117.28 0.077.19 0.057.14 0.067.19 0.047.02 0.076.88 0.117.32 0.117.07 0.056.82 0.157.01 0.097.32 0.096.71 0.267.51 0.24
C16:1, 9tNDNDNDNDNDNDNDNDNDNDNDNDNDNDNDND
C16:1, 9c0.74 0.020.71 0.030.69 0.020.72 0.040.72 0.040.70 .010.72 0.020.69 0.040.69 0.020.73 0.040.72 0.010.69 0.030.69 0.030.72 0.030.69 0.030.89 0.13
C18:01.28 0.041.28 0.051.25 0.051.31 0.021.17 0.091.31 0.051.31 0.051.31 0.051.25 0.051.31 0.011.30 0.021.28 0.021.27 0.061.28 0.001.32 0.041.51 0.09
C18:1, 6t/9t/11tNDNDNDNDNDNDNDNDNDNDNDNDNDNDNDND
C18:1, 9c28.02 0.1128.28 0.1128.13 0.0927.72 0.1327.68 0.1227.69 0.0727.59 0.1227.56 0.0328.13 0.0927.86 0.2327.85 0.0727.98 0.1428.13 0.0828.16 0.2227.96 0.1227.76 0.04
C18:2, 9t12tNDNDNDNDNDNDNDNDNDNDNDNDNDNDNDND
C18:2, 9c12tNDNDNDNDNDNDNDNDNDNDNDNDNDNDNDND
C18:2, 9t12cNDNDNDNDNDNDNDNDNDNDNDNDNDNDNDND
C18:2, 9c12c30.31 0.1230.47 0.0930.95 0.1630.88 0.0930.83 0.1830.41 0.3130.84 0.0330.77 0.1230.85 0.1630.67 0.1230.62 0.0630.67 0.0630.73 0.1430.62 0.0530.69 0.0729.04 0.08
C18:3, 9t12t15tNDNDNDNDNDNDNDNDNDNDNDNDNDNDND0.05 0.01
C18:3, 9t12t15c
or 9t12c15t
NDNDNDNDNDNDNDNDNDNDNDNDNDNDNDND
C18:3, 9c12t15t
or 9c12c15t
NDNDNDNDNDNDNDNDNDNDNDNDNDNDND0.09 0.01
C18:3, 9c12t15c
or 9t12c15c
NDNDNDNDNDNDNDNDNDNDNDNDNDNDNDND
C18:3, 9c12c15c32.44 0.1332.13 0.1732.11 0.2132.08 0.1832.43 0.1132.75 0.1132.35 0.2732.65 0.3032.20 0.2132.11 0.3132.43 0.2132.56 0.1532.17 0.2331.90 0.3132.63 0.2133.15 0.21
C22:1, tNDNDNDNDNDNDNDNDNDNDNDNDNDNDNDND
Subtotal trans0.00 0.000.00 0.000.00 0.000.00 0.000.00 0.000.00 0.000.00 0.000.00 0.000.00 0.000.00 0.000.00 0.000.00 0.000.00 0.000.00 0.000.00 0.000.14 0.02

Data are expressed as means ± SD from at least three independent experiments. ND means not detected.