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Journal of Chemistry
Volume 2016, Article ID 8570321, 9 pages
http://dx.doi.org/10.1155/2016/8570321
Research Article

The Coffee Protective Effect on Catalase System in the Preneoplastic Induced Rat Liver

1Exact Sciences Institute, Federal University of Alfenas, Rua Gabriel Monteiro da Silva 700, Centro, 37130-000 Alfenas, MG, Brazil
2Chemistry Institute, Federal University of Alfenas, Rua Gabriel Monteiro da Silva 700, Centro, 37130-000 Alfenas, MG, Brazil
3Nutrition Faculty, Federal University of Alfenas, Rua Gabriel Monteiro da Silva 700, Centro, 37130-000 Alfenas, MG, Brazil

Received 15 October 2015; Revised 2 March 2016; Accepted 28 March 2016

Academic Editor: Philippe Jeandet

Copyright © 2016 Cristiana Schmidt de Magalhães et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

This study aimed to evaluate the effect of organic/conventional coffee in liver tissues in the cancer process, taking into account the level and activities of catalase. The experiments were carried out with 8 groups of rats during 12 weeks. They received two injections of ethylenediaminetetraacetic acid solution 1.5% (v/v) prepared in 0.9% NaCl or 1,2-dimethylhydrazine (DMH) subcutaneous dose of 40 mg·kg−1·bw−1 for 2 weeks. The organic/conventional coffee infusions were at 5, 10, and 20% and were incorporated to feed (100 mL of infusion·kg−1 of diet). The catalase activity showed a decrease for livers which received DMH and DMH plus organic coffee at 5% and 10%. However, an increase was observed for those receiving organic 20% and conventional 10% coffee, slowing down and favoring the reversibility of the carcinogenic process. By SDS-PAGE, we observed an intensity decrease of 59 kDa bands, as the percentage of coffee was increased. The iron concentration (by ET-AAS) confirmed the electrophoretic results, suggesting that the DMH influenced the catalase expression conditions, reducing the activity by the loss of iron ions. Thus, the coffee may restore the catalase system in the liver, exerting its chemopreventive effects.