Comparison of Neuroprotective and Cognition-Enhancing Properties of Hydrolysates from Soybean, Walnut, and Peanut Protein

Table 2

Flow cytometry analysis for the neuroprotective effects of SPH, WPH, and PPH on H₂O₂-induced apoptosis in cultured PC12 cells.

Sample	Added amount (mg/mL)	Mechanically damaged cells (%)	Nonviable apoptotic cells (%)	Viable cells (%)	Viable apoptotic cells (%)
Control	—	7.75 ± 0.68	4.37 ± 0.32	65.91 ± 4.79	21.97 ± 2.15
H2O2	—	0.44 ± 0.12	24.63 ± 3.25	11.62 ± 2.61	63.31 ± 7.05
H2O2 + cerebrolysin	1	0.32 ± 0.11	23.29 ± 4.18	12.08 ± 1.49	64.31 ± 6.74
	2	0.32 ± 0.19	17.59 ± 1.02	18.08 ± 1.83	64.01 ± 10.08
	5	6.49 ± 0.76	70.08 ± 8.70	12.54 ± 2.04	10.89 ± 1.73
H2O2 + SPH	1	0.14 ± 0.05	25.74 ± 2.56	11.69 ± 1.87	63.43 ± 6.24
	2	0.12 ± 0.04	15.60 ± 3.01	22.07 ± 3.47	62.21 ± 7.22
	5	3.46 ± 0.95	75.69 ± 9.36	12.91 ± 2.26	7.94 ± 1.88
H2O2 + WPH	1	0.18 ± 0.06	25.25 ± 5.06	12.32 ± 1.69	62.25 ± 6.54
	2	0.25 ± 0.07	16.14 ± 1.89	19.02 ± 2.03	64.59 ± 6.01
	5	5.87 ± 0.84	76.57 ± 6.98	11.57 ± 1.21	5.99 ± 0.79
H2O2 + PPH	1	1.09 ± 0.42	22.87 ± 3.13	12.91 ± 2.07	63.13 ± 6.43
	2	0.16 ± 0.04	14.25 ± 2.78	15.14 ± 2.69	70.18 ± 7.22
	5	0.70 ± 0.10	62.66 ± 6.42	15.04 ± 3.02	21.60 ± 4.56

Each value indicates mean ± SD (). p < 0.01 and p < 0.001, compared with the H₂O₂-treated group.