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Journal of Chemistry
Volume 2017, Article ID 4357456, 13 pages
https://doi.org/10.1155/2017/4357456
Research Article

Difference between Burley Tobacco and Flue-Cured Tobacco in Nitrate Accumulation and Chemical Regulation of Nitrate and TSNA Contents

1Henan Agricultural University, National Tobacco Cultivation & Physiology & Biochemistry Research Center, Zhengzhou 450002, China
2Beijing Cigarette Factory of Shanghai Tobacco Group, Beijing 100024, China

Correspondence should be addressed to Hongzhi Shi; moc.361@ihsgnohihz

Received 11 July 2017; Accepted 6 November 2017; Published 6 December 2017

Academic Editor: Davide Vione

Copyright © 2017 Yafei Li et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

Tobacco-specific nitrosamines (TSNAs) are harmful carcinogens, with nitrate as a precursor of their formation. Nitrate content is considerably higher in burley tobacco than in flue-cured tobacco, but little has been reported on the differences between types of nitrate accumulation during development. We explored nitrate accumulation prior to harvest and examined the effects of regulatory substances aimed at decreasing nitrate and TSNA accumulation. In growth experiments, nitrate accumulation in burley and flue-cured tobacco initially increased but then declined with the highest nitrate content observed during a fast-growth period. When treating tobacco crops with molybdenum (Mo) during fast growth, nitrate reductase activity in burley tobacco increased significantly, but the NO3-N content decreased. These treatments also yielded significant reductions in NO3-N and TSNA contents. Therefore, we suggest that treatment with Mo during the fast-growth period and a Mo-Gfo (Mo-glufosinate) combination at the maturity stage is an effective strategy for decreasing nitrate and TSNAs during cultivation.