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Journal of Chemistry
Volume 2017, Article ID 6369358, 12 pages
Review Article

Measuring Antioxidant Activity in Bioorganic Samples by the Differential Oxygen Uptake Apparatus: Recent Advances

Department of Chemistry “G. Ciamician”, University of Bologna, Via S. Giacomo 11, 40126 Bologna, Italy

Correspondence should be addressed to Riccardo Amorati; ti.obinu@itaroma.odraccir

Received 2 January 2017; Accepted 27 February 2017; Published 19 March 2017

Academic Editor: Jacques Lalevee

Copyright © 2017 Riccardo Amorati et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.


The measure of O2 consumption during the inhibited autoxidation of an easily oxidizable substrate is one of the most reliable and predictive methods to assess antioxidant activity, especially for structure-activity relationship studies, for food and industrial applications. The differential oxygen uptake apparatus described herein represents a powerful and cost-effective way to obtain antioxidant activity from inhibited autoxidation studies. These experiments provide the rate constant and the stoichiometry of the reaction between antioxidants and peroxyl radicals (), which are involved in the propagation of radical damage. We show the operation principles and the utility of this instrumentation in the bioorganic laboratory, with regard to the recent advances in this field, ranging from the study of natural antioxidants in biomimetic system, to the use of substrates generating hydroperoxyl radicals, and to the evaluation of novel nanoantioxidants.