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Journal of Chemistry
Volume 2017, Article ID 6435086, 8 pages
Research Article

Simultaneous Determination of Caffeine and Chlorogenic Acids in Green Coffee by UV/Vis Spectroscopy

1Department of Physics and Chemistry, University of Palermo, Viale delle Scienze, Ed 18, 90128 Palermo, Italy
2Morettino Coffee Company, Angelo Morettino Srl, Via Enzo Biagi 3/5, 90146 Palermo, Italy
3Biophysics Institute, National Research Council, Via Ugo La Malfa 153, 90146 Palermo, Italy

Correspondence should be addressed to G. Navarra; moc.liamg@avanvoig

Received 10 May 2017; Accepted 25 September 2017; Published 26 October 2017

Academic Editor: Maria B. P. P. Oliveira

Copyright © 2017 G. Navarra et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.


A simple method for the simultaneous determination of caffeine and chlorogenic acids content in green coffee was reported. The method was based on the use of UV/Vis absorption. It is relevant that the quantification of both caffeine and chlorogenic acids was performed without their preliminary chemical separation despite their spectral overlap in the range 250–350 nm. Green coffee was extracted with 70% ethanol aqueous solution; then the solution was analyzed by spectroscopy. Quantitative determination was obtained analytically through deconvolution of the absorption spectrum and by applying the Lambert-Beer law. The bands used for the deconvolution were the absorption bands of both caffeine and chlorogenic acids standards. The molar extinction coefficients for caffeine and chlorogenic acid in ethanol solution at 70% were calculated by using the chemical standards; the estimated values were  M−1 cm−1 for caffeine and  M−1 cm−1 for chlorogenic acids molecules, respectively. The estimate of concentration values was in agreement with the one obtained by High Performance Liquid Chromatography quantification. The method is fast and simple and allows us to realize routine controls during the coffee production. In addition, it could be applied on roasted coffee and espresso coffee.