Simultaneous Determination of Caffeine and Chlorogenic Acids in Green Coffee by UV/Vis Spectroscopy

Table 1

Percent quantification of caffeine and CGAs into ground coffee of Arabica (A1, A2, and A3) and Robusta (R1 and R2) coffee as obtained by applying UV/Vis absorption and HPLC methods. Error associated with calculated concentration value was the standard deviation obtained from triplicate measurements.

Samples	% caffeine		% CGAs
	UV/Vis determination	HPLC determination	UV/Vis determination	HPLC determination
A1	0,94 ± 0,20	1,19 ± 0,25	6,17 ± 0,75	7,01 ± 0,85
A2	0,90 ± 0,05	0,95 ± 0,05	4,40 ± 0,50	4,96 ± 0,57
A3	1,09 ± 0,31	0,85 ± 0,24	5,89 ± 0,25	5,65 ± 0,24
R1	3,01 ± 0,53	3,65 ± 0,64	15,05 ± 0,09	15,15 ± 0,09
R2	1,66 ± 0,19	1,87 ± 0,21	5,89 ± 0,28	5,64 ± 0,26

ns: no significant difference (ANOVA).