Research Article
HPLC Quantification of Cytotoxic Compounds from Aspergillus niger
Table 2
Repeatability and intermediate precision data of the bioactive compounds 1–3 produced by A. niger (BRF 074).
| | Compound | Repeatability () | Intermediate precision () | Concentration
(µg mL−1) | Measured values
(µg mL−1) | Mean
(µg mL−1) | RSD
(%) | Concentration
(µg mL−1) | Measured values
(µg mL−1) | Mean
(µg mL−1) | RSD
(%) |
| | Furan ester (1) | 50.0 | 54.2892
54.3437
54.2872 | 54.3067 | 0.06 | 50.0 | 50.1338
50.2537
50.0912 | 50.1595 | 0.17 | | 250.0 | 233.8591
234.3952
234.1043 | 234.1195 | 0.11 | 250.0 | 245.7478
245.7127
245.7350 | 245.7319 | 0.01 | | 500.0 | 509.2980
509.2856
509.2549 | 509.2795 | 0.01 | 500.0 | 500.9200
500.8662
501.3840 | 501.0567 | 0.06 |
| | Malformin A (2) | 50.0 | 51.7388
51.7246
51.6044 | 51.6893 | 0.14 | 50.0 | 49.3617
49.4094
49.4188 | 49.3967 | 0.06 | | 250.0 | 246.2154
246.0825
246.1936 | 246.1638 | 0.03 | 250.0 | 239.8196
240.0045
240.0410 | 239.9550 | 0.05 | | 500.0 | 503.6637
502.9370
503.2363 | 503.2790 | 0.07 | 500.0 | 505.5801
505.6485
505.8089 | 505.6792 | 0.02 |
| | Malformin C (3) | 50.0 | 52.8589
52.7992
52.8364 | 52.8315 | 0.06 | 50.0 | 51.1270
51.0972
50.9613 | 51.0618 | 0.17 | | 250.0 | 241.1501
241.3867
241.4114 | 241.3161 | 0.06 | 250.0 | 231.0606
231.2140
231.3173 | 231.1973 | 0.06 | | 500.0 | 505.8446
506.9641
505.1826 | 505.9971 | 0.18 | 500.0 | 510.3529
510.2716
510.1154 | 510.2467 | 0.02 |
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