Interspecies In Vitro Evaluation of Stereoselective Protein Binding for 3,4-Methylenedioxymethamphetamine

Table 2

Percentage of MDMA enantiomers bound to protein in serum or plasma according to species.

Species	Concentration of rac-MDMA (ng/mL)	Compound	Mean peak area ratios		Percentage of enantiomer bound to protein (%)	Enantiomer ratio (R) versus (S)	Total MDMA bound to protein (%)
			Sample ()	Control ()
Human (serum)	20	(−)-(R)-MDMA		0.089	41	0.94	43
		(+)-(S)-MDMA		0.096	44
	200	(−)-(R)-MDMA		0.865	51	0.99	51
		(+)-(S)-MDMA		0.847	51
Rat (plasma)	20	(−)-(R)-MDMA		0.073	38	0.92	39
		(+)-(S)-MDMA		0.078	41
	200	(−)-(R)-MDMA		0.843	47	0.93	49
		(+)-(S)-MDMA		0.861	50
Mouse (plasma)	20	(−)-(R)-MDMA		0.088	28	0.78	32
		(+)-(S)-MDMA		0.084	36
	200	(−)-(R)-MDMA		0.836	11	0.74	13
		(+)-(S)-MDMA		0.850	15