Transglycosylation Properties of a Novel α-1,4-Glucanotransferase from Bacteroides thetaiotaomicron and Its Application in Developing an α-Glucosidase-Specific Inhibitor

Table 1

Purification of the BtαGTase enzyme.

Step	Volume (mL)	Enzyme activity (U)	Protein concentration (mg/mL)	Protein amount (mg)	Specific activity (U/mg)	Yield (%)	Purification fold
Cell extract	104	3.57	169.44	17621.76	0.20 × 10^−3	100	1.00
Ni-NTA	1.5	2.03	2.26	3.39	0.59	56.8	2953.20