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Journal of Chemistry
Volume 2018, Article ID 3082026, 10 pages
https://doi.org/10.1155/2018/3082026
Research Article

Acetylation Modification Improves Immunoregulatory Effect of Polysaccharide from Seeds of Plantago asiatica L.

State Key Laboratory of Food Science and Technology, Nanchang University, Nanchang 330047, China

Correspondence should be addressed to Shao-Ping Nie; nc.ude.ucn@einps and Ming-Yong Xie; nc.ude.ucn@eixym

Received 17 October 2017; Revised 13 December 2017; Accepted 8 January 2018; Published 13 February 2018

Academic Editor: Ji Kang

Copyright © 2018 Le-Ming Jiang et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

The current study was conducted to investigate effects of acetylated Plantago asiatica L. polysaccharides (Ac-PLCPs) on their immunoregulatory activities in bone-marrow-derived dendritic cells (DCs) model. Influences of natural Plantago asiatica L. crude polysaccharide (PLCP) and Ac-PLCPs on inducing phenotypic and functional maturation on DCs were determined. The results showed that Ac-PLCPs with degree of substitution (DS) from 0.06 to 0.1 could not only stimulate the expression of surface molecules major histocompatibility complex class II (MHC II), cluster of differentiation 86 (CD86), and CD80 on DCs but also increase the secretion of cytokine IL-12p70 . The endocytosis activity of DCs was attenuated by Ac-PLCPs treatment , while the mRNA expressions of chemokine receptors CCR7 and CXCR4 in DCs were significantly increased . Besides, DCs treated with the Ac-PLCPs showed extremely strong T cell proliferation stimulating activity . These data showed that Ac-PLCPs had higher maturation-stimulating activity on DCs than PLCP, which indicated that acetylation modification improved the immunoregulatory effect of PLCP.