Simultaneous Determination of Pesticides in Fruits by Using Second-Order Fluorescence Data Resolved by Unfolded Partial Least-Squares Coupled to Residual Bilinearization

Table 6

Calibration in presence of matrix: real sample analysis by applying methanolic extraction and UPLS/RBL. Comparison with a reference method based on HPLC being: calculated t-values = 1.48, degrees of freedom n = 13, and the corresponding tabulated critical t‐ values = 2.16 (α = 0.05/2).

Samples	Carbaryl^†§			Carbendazim^†§			Thiabendazole^†§
	Proposed method		Reference method	Proposed method		Reference method	Proposed method		Reference method
	µg·L^−1	mg·kg^−1	mg·kg^−1	µg·L^−1	mg·kg^−1	mg·kg^−1	µg·L^−1	mg·kg^−1	mg·kg^−1
B1	—	—	—	—	—	—	4.1 (1)	0.076	0.070
B2	—	—	—	4.0 (1)	0.080	0.087	7.0 (2)	0.14	0.13
B3	—	—	—	5.1 (1)	0.100	0.090	4.0 (2)	0.080	0.089
B4	—	—	—	—	—	—	—	—	—
B5	—	—	—	6.0 (1)	0.120	0.112	4.1 (1)	0.080	0.091
N1	10.1 (1)	0.200	0.189	—	—	—	—	—	—
N2	15.3 (3)	0.300	0.330	—	—	—	—	—	—
N3	5.2 (1)	0.100	0.110	—	—	—	1.1 (1)	0.018	0.025
N4	1.1 (1)	0.018	0.024	—	—	—	24.0 (2)	0.480	0.500

^†Average of three determinations. Standard deviation in parenthesis obtained by three replicate analyses. N_u: number of unexpected components (1 to 3) depending on samples. ^§Predicted concentration values statistically compared with HPLC values for all pesticides in both fruits, applying paired t-test: being: calculated t-values = 1.48, degrees of freedom ; and the corresponding tabulated critical t-values = 2.16 (α = 0.05/2).