(a)
(b)
(c)
(d)
Figure 1: Effect of γ-tocotrienol on viability in MC3T3-E1 cells. (a) The structure of tocopherol and tocotrienol. (b) Cells were exposed to different doses of γ-tocotrienol for 24, 48, or 72 h. Cell viability was determined by MTT assay. (c) Cells were treated with different doses of γ-tocotrienol for 24, 48, and 72 h. Images were captured by phase contrast microscopy (200x). (d) Cells were stained with DAPI dye for measuring nuclear morphological alteration (DAPI, 200x). Data are expressed as mean ± SD (). , compared to the control group. B: blank control; C: ethanol control.