Figure 3: Effect of γ-tocotrienol on the differentiation in MC3T3-E1 cells. Cells were treated with γ-tocotrienol at different doses for the desired time. The ALP activity (a), OC and ON amount ((b), (c)), and Col I mRNA level (d) were determined by colorimetric analysis, ELISA, and real-time PCR analysis, respectively. Data are expressed as mean ± SD (). and , compared to the control group. B: blank control; C: ethanol control.