Figure 4: Effect of γ-tocotrienol on mRNA and protein levels of Runx2 in MC3T3-E1 cells. Cells were treated with different doses of γ-tocotrienol for 72 h. The mRNA (a) and protein expression (b) of Runx2 were determined by real-time PCR and Western blot. Data are expressed as mean ± SD (). , compared to the control group. B: blank control; C: ethanol control.