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Journal of Chemistry
Volume 2018, Article ID 3805932, 9 pages
https://doi.org/10.1155/2018/3805932
Research Article

Gamma-Tocotrienol Stimulates the Proliferation, Differentiation, and Mineralization in Osteoblastic MC3T3-E1 Cells

1Department of Food Science and Engineering, School of Chemistry and Chemical Engineering, Harbin Institute of Technology, Harbin 150001, China
2Department of Central Laboratory, The First Affiliated Hospital of Harbin Medical University, Harbin 150090, China
3School of Life Science, Institute of Biomedical and Environmental Science and Technology, University of Bedfordshire, Luton LU1 3JU, UK

Correspondence should be addressed to Weili Xu; moc.anis@7791uxiliew and Shaobo Zhou; ku.ca.sdeb@uohz.oboahs

Received 11 October 2017; Accepted 6 December 2017; Published 15 January 2018

Academic Editor: Ji Kang

Copyright © 2018 Weili Xu et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Linked References

  1. S. Khosla, J. J. Westendorf, and M. J. Oursler, “Building bone to reverse osteoporosis and repair fractures,” The Journal of Clinical Investigation, vol. 118, no. 2, pp. 421–428, 2008. View at Publisher · View at Google Scholar · View at Scopus
  2. Y.-C. Lu, Y. C. Lin, Y.-K. Lin et al., “Prevalence of osteoporosis and low bone mass in older chinese population based on bone mineral density at multiple skeletal sites,” Scientific Reports, vol. 6, Article ID 25206, 2016. View at Publisher · View at Google Scholar · View at Scopus
  3. P. Sambrook and C. Cooper, “Osteoporosis,” The Lancet, vol. 367, no. 9527, pp. 2010–2018, 2006. View at Publisher · View at Google Scholar · View at Scopus
  4. H. Hoshino, M. Takahashi, K. Kushida, T. Ohishi, and T. Inoue, “The relationships between the degree of β-isomerization of type I collagen degradation products in the urine and aging, menopause and osteoporosis with fractures,” Osteoporosis International, vol. 9, no. 5, pp. 405–409, 1999. View at Publisher · View at Google Scholar · View at Scopus
  5. I. R. Reid, “Anti-resorptive therapies for osteoporosis,” Seminars in Cell & Developmental Biology, vol. 19, no. 5, pp. 473–478, 2008. View at Publisher · View at Google Scholar · View at Scopus
  6. G. A. Rodan and T. J. Martin, “Therapeutic approaches to bone diseases,” Science, vol. 289, no. 5484, pp. 1508–1514, 2000. View at Publisher · View at Google Scholar · View at Scopus
  7. B. L. Riggs and A. M. Parfitt, “Drugs used to treat osteoporosis: the critical need for a uniform nomenclature based on their action on bone remodeling,” Journal of Bone and Mineral Research, vol. 20, no. 2, pp. 177–184, 2005. View at Publisher · View at Google Scholar · View at Scopus
  8. N. E. Lane and A. Kelman, “Review of anabolic therapies for osteoporosis,” Arthritis Research & Therapy, vol. 5, pp. 214–222, 2003. View at Google Scholar
  9. P. Ducy, T. Schinke, and G. Karsenty, “The osteoblast: a sophisticated fibroblast under central surveillance,” Science, vol. 289, no. 5484, pp. 1501–1504, 2000. View at Publisher · View at Google Scholar · View at Scopus
  10. L. Packer, S. U. Weber, and G. Rimbach, “Molecular aspects of alpha-tocotrienol antioxidant action and cell signalling,” Journal of Nutrition, vol. 131, pp. 369S–373S, 2001. View at Google Scholar
  11. P. J. McLaughlin and J. L. Weihrauch, “Vitamin E content of foods,” Journal of The American Dietetic Association, vol. 75, pp. 647–665, 1979. View at Google Scholar
  12. N. Miyoshi, Y. Wakao, S. Tomono, M. Tatemichi, T. Yano, and H. Ohshima, “The enhancement of the oral bioavailability of gamma-tocotrienol in mice by gamma-cyclodextrin inclusion,” The Journal of Nutritional Biochemistry, vol. 22, no. 12, pp. 1121–1126, 2011. View at Publisher · View at Google Scholar · View at Scopus
  13. A. S. Nazrun, M. Norazlina, M. Norliza, and S. Ima Nirwana, “Comparison of the effects of tocopherol and tocotrienol on osteoporosis in animal models,” International Journal of Pharmacology, vol. 6, no. 5, pp. 561–568, 2010. View at Publisher · View at Google Scholar · View at Scopus
  14. M. Z. Mehat, A. N. Shuid, N. Mohamed, N. Muhammad, and I. N. Soelaiman, “Beneficial effects of vitamin e isomer supplementation on static and dynamic bone histomorphometry parameters in normal male rats,” Journal of Bone and Mineral Metabolism, vol. 28, no. 5, pp. 503–509, 2010. View at Publisher · View at Google Scholar · View at Scopus
  15. M. Norazlina, P. L. Lee, H. I. Lukman, A. S. Nazrun, and S. Ima-Nirwana, “Effects of vitamin E supplementation on bone metabolism in nicotine-treated rats,” Singapore Medical Journal, vol. 48, pp. 195–199, 2007. View at Google Scholar
  16. H. Hermizi, O. Faizah, S. Ima-Nirwana, S. Ahmad Nazrun, and M. Norazlina, “Beneficial effects of tocotrienol and tocopherol on bone histomorphometric parameters in Sprague-Dawley male rats after nicotine cessation,” Calcified Tissue International, vol. 84, no. 1, pp. 65–74, 2009. View at Publisher · View at Google Scholar · View at Scopus
  17. N. S. Ahmad, B. A. K. Khalid, D. A. Luke, and S. I. Nirwana, “Tocotrienol offers better protection than tocopherol from free radical-induced damage of rat bone,” Clinical and Experimental Pharmacology and Physiology, vol. 32, no. 9, pp. 761–770, 2005. View at Publisher · View at Google Scholar · View at Scopus
  18. S. Maniam, N. Mohamed, A. N. Shuid, and I. N. Soelaiman, “Palm tocotrienol exerted better antioxidant activities in bone than α-tocopherol,” Basic & Clinical Pharmacology & Toxicology, vol. 103, no. 1, pp. 55–60, 2008. View at Publisher · View at Google Scholar · View at Scopus
  19. S. Abdul-Majeed, N. Mohamed, and I.-N. Soelaiman, “The use of delta-tocotrienol and lovastatin for anti-osteoporotic therapy,” Life Sciences, vol. 125, pp. 42–48, 2015. View at Publisher · View at Google Scholar
  20. S. Ima-Nirwana, A. Kiftiah, A. G. Zainal, M. Norazlina, M. T. Gapor, and B. A. K. Khalid, “Palm vitamin E prevents osteoporosis in orchidectomized growing male rats,” Natural Product Sciences, vol. 6, no. 4, pp. 155–160, 2000. View at Google Scholar · View at Scopus
  21. M. Norazlina, S. Ima-Nirwana, M. T. Gapor, and B. A. K. Khalid, “Palm vitamin E is comparable to α-tocopherol in maintaining bone mineral density in ovariectomised female rats,” Experimental and Clinical Endocrinology & Diabetes, vol. 108, no. 4, pp. 305–310, 2000. View at Publisher · View at Google Scholar · View at Scopus
  22. S. Ima-Nirwana and S. Suhaniza, “Effects of Tocopherols and Tocotrienols on Body Composition and Bone Calcium Content in Adrenalectomized Rats Replaced with Dexamethasone,” Journal of Medicinal Food, vol. 7, no. 1, pp. 45–51, 2004. View at Publisher · View at Google Scholar · View at Scopus
  23. A. S. Nazrun, D. A. Luke, B. A. K. Khalid, and S. Ima-Nirwana, “Vitamin E protects from free-radical damage on femur of rats treated with ferric nitrilotriacetate,” Current Topics in Pharmacology, vol. 9, pp. 107–115, 2005. View at Google Scholar
  24. I. N. Soelaiman, W. Ming, R. Abu Bakar et al., “Palm tocotrienol supplementation enhanced bone formation in oestrogen-deficient rats,” International Journal of Endocrinology, vol. 2012, Article ID 532862, 7 pages, 2012. View at Publisher · View at Google Scholar · View at Scopus
  25. A. N. Shuid, Z. Mehat, N. Mohamed, N. Muhammad, and I. N. Soelaiman, “Vitamin E exhibits bone anabolic actions in normal male rats,” Journal of Bone and Mineral Metabolism, vol. 28, no. 2, pp. 149–156, 2010. View at Publisher · View at Google Scholar · View at Scopus
  26. A. M. Nizar, A. S. Nazrun, M. Norazlina, M. Norliza, and S. Ima Nirwana, “Low dose of tocotrienols protects osteoblasts against oxidative stress,” La Clinica Terapeutica, vol. 162, no. 6, pp. 533–538, 2011. View at Google Scholar · View at Scopus
  27. K.-Y. Chin and S. Ima-Nirwana, “The biological effects of tocotrienol on bone: A review on evidence from rodent models,” Drug Design, Development and Therapy, vol. 9, pp. 2049–2061, 2015. View at Publisher · View at Google Scholar · View at Scopus
  28. P. Chiewwei, C. Yuenmay, A. N. Ma, and C. Chenghock, “The effect of physical refining on palm vitamin E (tocopherol, tocotrienol and tocomonoenol,” American Journal of Applied Sciences, vol. 4, pp. 374–377, 2007. View at Google Scholar
  29. L. Deng, Y. Ding, Y. Peng et al., “γ-Tocotrienol protects against ovariectomy-induced bone loss via mevalonate pathway as HMG-CoA reductase inhibitor,” Bone, vol. 67, pp. 200–207, 2014. View at Publisher · View at Google Scholar · View at Scopus
  30. W. Xu, M. Du, Y. Zhao, Q. Wang, W. Sun, and B. Chen, “γ-Tocotrienol inhibits cell viability through suppression of β-catenin/Tcf signaling in human colon carcinoma HT-29 cells,” The Journal of Nutritional Biochemistry, vol. 23, no. 7, pp. 800–807, 2012. View at Publisher · View at Google Scholar · View at Scopus
  31. K. J. Livak and T. D. Schmittgen, “Analysis of relative gene expression data using real-time quantitative PCR and the 2(-Delta Delta C (T)) method,” Methods, vol. 25, no. 4, pp. 402–408, 2001. View at Publisher · View at Google Scholar · View at Scopus
  32. Y. Gao, Effects of icariin on the proliferation and differentiation of osteoblast and its possible mechanism of action in vitro [Ph.D. thesis], Hebei Medical University, China, 2013.
  33. A. Steinbach, A. Tautzenberger, A. Ignatius, M. Pluntke, O. Marti, and D. Volkmer, “Coatings from micropatterned sulfobetaine polymer brushes as substrates for MC3T3-E1 cells,” Journal of Materials Science: Materials in Medicine, vol. 23, no. 2, pp. 573–579, 2012. View at Publisher · View at Google Scholar · View at Scopus
  34. M.-B. Kim, Y. Song, and J.-K. Hwang, “Kirenol stimulates osteoblast differentiation through activation of the BMP and Wnt/β-catenin signaling pathways in MC3T3-E1 cells,” Fitoterapia, vol. 98, pp. 59–65, 2014. View at Publisher · View at Google Scholar · View at Scopus
  35. M.-T. Tsai, D.-J. Lin, S. Huang, H.-T. Lin, and W. H. Chang, “Osteogenic differentiation is synergistically influenced by osteoinductive treatment and direct cell-cell contact between murine osteoblasts and mesenchymal stem cells,” International Orthopaedics, vol. 36, no. 1, pp. 199–205, 2012. View at Publisher · View at Google Scholar · View at Scopus
  36. W.-L. Xu, J.-R. Liu, H.-K. Liu et al., “Inhibition of proliferation and induction of apoptosis by γ-tocotrienol in human colon carcinoma HT-29 cells,” Nutrition Journal , vol. 25, no. 5, pp. 555–566, 2009. View at Publisher · View at Google Scholar · View at Scopus
  37. N. Abd Manan, N. Mohamed, and A. N. Shuid, “Effects of low-dose versus high-dose γ -tocotrienol on the bone cells exposed to the hydrogen peroxide-induced oxidative stress and apoptosis,” Evidence-Based Complementary and Alternative Medicine, vol. 2012, Article ID 680834, 10 pages, 2012. View at Publisher · View at Google Scholar · View at Scopus
  38. R. Pazdro and J. R. Burgess, “The role of vitamin E and oxidative stress in diabetes complications,” Mechanisms of Ageing and Development, vol. 131, no. 4, pp. 276–286, 2010. View at Publisher · View at Google Scholar · View at Scopus
  39. E. Serbinova, V. Kagan, D. Han, and L. Packer, “Free radical recycling and intramembrane mobility in the antioxidant properties of alpha-tocopherol and alpha-tocotrienol,” Free Radical Biology & Medicine, vol. 10, no. 5, pp. 263–275, 1991. View at Publisher · View at Google Scholar · View at Scopus
  40. Z. P. Barnouti, P. Owtad, G. Shen, P. Petocz, and M. A. Darendeliler, “The biological mechanisms of PCNA and BMP in TMJ adaptive remodeling.,” The Angle Orthodontist, vol. 81, no. 1, pp. 91–99, 2011. View at Publisher · View at Google Scholar · View at Scopus
  41. S. E. Johnson and R. E. Allen, “Proliferating cell nuclear antigen (PCNA) is expressed in activated rat skeletal muscle satellite cells,” Journal of Cellular Physiology, vol. 154, no. 1, pp. 39–43, 1993. View at Publisher · View at Google Scholar · View at Scopus
  42. D. Mccormick, H. Chong, C. Hobbs, C. Datta, and P. A. Hall, “Detection of the Ki‐67 antigen in fixed and wax‐embedded sections with the monoclonal antibody MIB1,” Histopathology, vol. 22, no. 4, pp. 355–360, 1993. View at Publisher · View at Google Scholar · View at Scopus
  43. W. N. Addison, F. Azari, E. S. Sørensen, M. T. Kaartinen, and M. D. McKee, “Pyrophosphate inhibits mineralization of osteoblast cultures by binding to mineral, up-regulating osteopontin, and inhibiting alkaline phosphatase activity,” The Journal of Biological Chemistry, vol. 282, no. 21, pp. 15872–15883, 2007. View at Publisher · View at Google Scholar · View at Scopus
  44. K. H. Wlodarski, “Properties and Origin of Osteoblasts,” Clinical Orthopaedics and Related Research, vol. 252, pp. 276–293, 1990. View at Google Scholar
  45. Y.-F. Chou, J. C. Y. Dunn, and B. M. Wu, “In vitro response of MC3T3-E1 preosteoblasts within three-dimensional apatite-coated PLGA scaffolds,” Journal of Biomedical Materials Research Part B: Applied Biomaterials, vol. 75, no. 1, pp. 81–90, 2005. View at Publisher · View at Google Scholar · View at Scopus
  46. G. Jundt, K.-H. Berghäuser, J. D. Termine, and A. Schulz, “Osteonectin—a differentiation marker of bone cells,” Cell and Tissue Research, vol. 248, no. 2, pp. 409–415, 1987. View at Publisher · View at Google Scholar · View at Scopus
  47. M. Jagodzinski, M. Drescher, J. Zeichen et al., “Effects of cyclic longitudinal mechanical strain and dexamethasone on osteogenic differentiation of human bone marrow stromal cells,” European Cells and Materials, vol. 7, pp. 35–41, 2004. View at Publisher · View at Google Scholar · View at Scopus
  48. P. Clement-Lacroix, M. Ai, F. Morvan et al., “Lrp5-independent activation of Wnt signaling by lithium chloride increases bone formation and bone mass in mice,” Proceedings of the National Acadamy of Sciences of the United States of America, vol. 102, no. 48, pp. 17406–17411, 2005. View at Publisher · View at Google Scholar · View at Scopus
  49. L.-S. Song, Z.-X. Zhang, Y. Wang, Y. Liu, R. Zhang, and L.-J. Lu, “Effects of nano-emulsion preparations of tocopherols and tocotrienols on oxidative stress and osteoblast differentiation,” Archives of Biological Sciences, vol. 69, no. 1, pp. 149–156, 2017. View at Publisher · View at Google Scholar · View at Scopus
  50. P. Ducy, R. Zhang, V. Geoffroy, A. L. Ridall, and G. Karsenty, “Osf2/Cbfa1: a transcriptional activator of osteoblast differentiation,” Cell, vol. 89, no. 5, pp. 747–754, 1997. View at Publisher · View at Google Scholar · View at Scopus
  51. P. Ducy, “Cbfa1: A molecular switch in osteoblast biology,” Developmental Dynamics, vol. 219, no. 4, pp. 461–471, 2000. View at Publisher · View at Google Scholar · View at Scopus
  52. K. Nakashima, X. Zhou, G. Kunkel et al., “The novel zinc finger-containing transcription factor Osterix is required for osteoblast differentiation and bone formation,” Cell, vol. 108, no. 1, pp. 17–29, 2002. View at Publisher · View at Google Scholar · View at Scopus
  53. C. Wang, M.-X. Meng, X.-L. Tang et al., “The proliferation, differentiation, and mineralization effects of puerarin on osteoblasts in vitro,” Chinese Journal of Natural Medicines, vol. 12, no. 6, pp. 436–442, 2014. View at Publisher · View at Google Scholar · View at Scopus