Research Article
Relevance and Standardization of In Vitro Antioxidant Assays: ABTS, DPPH, and Folin–Ciocalteu
Table 1
The range of concentration of antioxidants in assay solution (cantioxidant).
| Sample | cantioxidant range (μmol/L) | DPPH | ABTS+ | FC | MeOH | H2O | pH 5 | pH 7.4 | H2O | pH 5 | pH 7.4 |
| CA | 8–40 | 8–40 | 8–40 | 3–15 | 8–40 | 8–40 | 8–40 | 3–15 | CGA | 6–30 | 6–30 | 6–30 | 2–10 | 6–30 | 6–30 | 4–20 | 6–30 | GA | 2.6–13 | 2.6–13 | 2.6–13 | 2.6–13 | 1.6–8.0 | 1.6–8.0 | 1.6–8.0 | 6–30 | CTH | 1.6–6.4 | 3–15 | 3–15 | 1.6–8 | 1.6–6.4 | 1.6–6.4 | 1.6–6.4 | 3–15 | EGCG | 1.4–7 | 1.4–7 | 1.4–7 | 0.8–4 | 1.4–7 | 0.8–4 | 0.8–4 | 3–15 | AA | 4–40 | 4–40 | 4–40 | 4–40 | 4–40 | 4–40 | 4–40 | 4–40 | DHA | 100–500 | 100–500 | 100–500 | 20–200 | 100–500 | 100–500 | 20–200 | 10–100 | Trolox | 5–35 | 5–35 | 5–35 | 5–35 | 5–35 | 5–35 | 5–35 | 5–35 | FeSO4 | — | — | — | — | — | — | — | 20–120 |
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